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TRANSGENIC RESEARCH The production of male-sterile wheat plants through split barnase expression is promoted by the insertion of introns and flexible peptide linkers Katja Kempe, Myroslava Rubtsova, David Riewe and Mario Gils
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TRANSGENIC RESEARCH The production of male-sterile wheat plants through split barnase expression is promoted by the insertion of introns and flexible peptide linkers Katja Kempe, Myroslava Rubtsova, David Riewe and Mario Gils LeibnizInstitute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstraße 3, 06466 Stadt Seeland OT Gatersleben, Germany gils@ipk-gatersleben.de - Supplemental Figure 2 - - Linker - Intron 1 Linker - Intron - Linker - Intron 1 Linker - Intron 1+5 1+6 1+5 1+6 2+6 2+6 2+7 1+7 1+5 2 Linker - Intron 2 Linker + Intron 2 Linker - Intron 1 Linker + Intron UBQ10-i1 [UTR] RB LB 5. pICH24431 1. pHW21 P35S Bar-N IntN Tocs RB LB (GGGGS)3 (GGGGS)3 6. pHW24612 2. pHW331 P35S Bar-N IntN Tocs (GGGGS)3 7. pHW701 RB RB RB LB LB LB Pact Pact Pact IntC IntC IntC Bar-C Bar-C Bar-C Tnos Tnos Tnos