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Unit 1 - Lecture 1 Microscope Objectives

Unit 1 - Lecture 1 Microscope Objectives 1-1 Understand that microbiology includes a diversity of microorganisms requires the regular use of the microscope. 1-2 Be able to use correct microscope technique, including the use of the oil immersion lens. Types of Microscopes.

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Unit 1 - Lecture 1 Microscope Objectives

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  1. Unit 1 - Lecture 1 Microscope Objectives 1-1 Understand that microbiology includes a diversity of microorganisms requires the regular use of the microscope. 1-2 Be able to use correct microscope technique, including the use of the oil immersion lens.

  2. Types of Microscopes Different types of microscopes 1. Simple - single lens (10-20X) (perfected by Anton van Leeuwenhoek) 2. Compound - 2 lens (year = 1590) 3. Compound light - (2,000X) 4. Stereomicroscope - used for large specimens (60X)

  3. 4. Electron - uses a beam of electrons instead of light (1932) a. Transmission - T.E.M. shows internal structures (200,000X) b.Scanning - S.E.M. shows the surface (100,000X)

  4. Microscope Do’s and Don’t’s How to Store: - remove cover/ replace cover - wrap cord around base - make sure stage is all the way up - make sure shortest lens is toward stage

  5. Other information small(4X) medium(10X) large - high (40X) 4 X 10 10 X 10 10 X 40 Total magnification = 40 = 100 = 400 - resolution - ability to distinguish between 2 objects - magnification - apparent increase in size of the organism - microscope makes a virtual image - upside down and backwards

  6. View Diameters 40 – 4.45 mm 100 – 1.78 mm 400 - .45 mm 1000 - .178 mm

  7. scanning electron microscope website

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