1 / 20

CHMI 2227E Biochemistry I

CHMI 2227E Biochemistry I. Enzymes: Basic concepts. Enzymes. Enzymes. Classes of enzymes. Example: ATP: D-glucose-6 phosphotransferase (aka hexokinase). EC 2.7.1.1 2 = transferase 7 = phosphotransferase 1 = acceptor group is OH 1 = glucose binds the phosphate group. ATP. +.

cynthialee
Download Presentation

CHMI 2227E Biochemistry I

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. CHMI 2227EBiochemistry I Enzymes: Basic concepts CHMI 2227 - E.R. Gauthier, Ph.D.

  2. Enzymes CHMI 2227 - E.R. Gauthier, Ph.D.

  3. Enzymes CHMI 2227 - E.R. Gauthier, Ph.D.

  4. Classes of enzymes CHMI 2227 - E.R. Gauthier, Ph.D.

  5. Example: ATP: D-glucose-6 phosphotransferase (aka hexokinase) EC 2.7.1.1 2 = transferase 7 = phosphotransferase 1 = acceptor group is OH 1 = glucose binds the phosphate group ATP + D-glucose ADP + D-glucose-6-phosphate IUBMB enzyme nomenclature CHMI 2227 - E.R. Gauthier, Ph.D.

  6. 1- Oxidoreductases 2- Transferases 3- Hydrolases 4- Lyases 5- Isomerases 6- Ligases Classes of enzymes CHMI 2227 - E.R. Gauthier, Ph.D.

  7. A- + B A + B- A-B + C A + B-C A-B + H2O A-H + B-OH Y X X Y Y X A=B + X-Y A-B A-B A-B A + B A-B Classes of enzymes • 1- Oxidoreductases • 2- Transferases • 3- Hydrolases • 4- Lyases • 5- Isomerases • 6- Ligases CHMI 2227 - E.R. Gauthier, Ph.D.

  8. OxidoreductasesExample: Lactate dehydrogenase (EC 1.1.1.27) • Lactate = substrate • Pyruvate = product • NAD+/NADH = co-factor CHMI 2227 - E.R. Gauthier, Ph.D.

  9. 2. TransferasesExample: Alanine transaminase (EC 2.6.1.2) • Alanine / a-ketoglutarate = substrates • Pyruvate / L-glutamate = products CHMI 2227 - E.R. Gauthier, Ph.D.

  10. 3. HydrolasesExample: diphosphate phosphohydrolase(EC 3.6.1.1) • Pyrophosphate / H2O = substrates • Phosphate = product CHMI 2227 - E.R. Gauthier, Ph.D.

  11. 4. Lyases (aka synthases)Example: Pyruvate decarboxylase(EC 4.1.1.1) • Pyruvate = substrate • Acetaldehyde and CO2 = products CHMI 2227 - E.R. Gauthier, Ph.D.

  12. 5. IsomerasesExample: Alanine racemase(EC 5.1.1.1) • L-alanine = substrate • D-alanine = product CHMI 2227 - E.R. Gauthier, Ph.D.

  13. 6. Ligases (aka synthetases)Example: L-glutamine synthetase(EC 6.3.1.2) • L-glutamate / NH4+ = substrates • L-glutamine = product • ATP = co-factor CHMI 2227 - E.R. Gauthier, Ph.D.

  14. How do enzymes work? CHMI 2227 - E.R. Gauthier, Ph.D.

  15. Enzymes and the energy barrier CHMI 2227 - E.R. Gauthier, Ph.D.

  16. DGŧ DG Enzymes and the energy barrier CHMI 2227 - E.R. Gauthier, Ph.D.

  17. Enzymes and the energy barrier CHMI 2227 - E.R. Gauthier, Ph.D.

  18. Enzymes and the energy barrier CHMI 2227 - E.R. Gauthier, Ph.D.

  19. The « lock and key » myth… CHMI 2227 - E.R. Gauthier, Ph.D.

  20. The « lock and key » myth… CHMI 2227 - E.R. Gauthier, Ph.D.

More Related