Plasma Membrane & Cellular Transport

1. Plasma Membrane & Cellular Transport http://www.i-sup2008.org/images/venue_transport.jpg

2. Cell Transport A cell has to move food and wastes into and out of the cell. Materials must move through the plasma membrane which maintains homeostasis in the cell. food food food waste waste waste waste food

3. Cell Transport The Plasma Membrane surrounds the cell. How does it work?

4. Plasma Membrane Boundary between the cell and its environment • Allows nutrients into the cell • Removes wastes and excess materials • Maintains homeostasis: a stable internal environment

5. Plasma Membrane How does it work? • Semi-permeable: only allows some molecules in the cell, keeps others out http://media3.washingtonpost.com/wp-dyn/content/photo/2006/10/15/PH2006101500491.jpg

6. Plasma Membrane 2. Fluid Mosaic Model: membrane is flexible, made of many pieces working together http://www.youtube.com/watch?v=Qqsf_UJcfBc

7. Plasma Membrane 3. PhospholipidBilayer: membrane is 2 layers, made of phosphates and fats (lipids) with proteins mixed in

8. Plasma Membrane Parts Phosphate heads – hydrophilic (like water), outside and inside membrane Fatty Acid Tails – hydrophobic (fear water), inside membrane, like OREO cream Cholesterol – prevents fats from sticking together, stabilize membrane Transport proteins – move molecules into and out of cell Identification proteins – outside cell, “nametag” Support proteins – inside cell for framework, “skeleton”

9. Membrane Model Lab Purpose: What are the parts of a plasma membrane? Arrange your membrane parts to look like this section of membrane. On your paper towel, label the inside and outside of the cell. Marshmallows = phosphates Toothpicks = fatty acid chains Gummy Worms / Twizzlers= proteins Candy Corn= cholesterol Inside cell #3 #1 #2 Outside cell

10. Membrane Model Lab • Draw model in your notes. Label proteins, lipids, phosphates, cholesterol, outside and inside of cell, hydrophobic portion. • What type of protein is each of the numbered arrows? How is each used? • Why are the marshmallows not attached to each other? • Where would cholesterol be found in the membrane? Why?

11. Model a membrane • Using bubble solution, show a flexible membrane. Why is this important? • Form an opening in the membrane with a circle of string, pop the inside. This is how a channel protein works. Move it around in the membrane. (Membrane is fluid) • What happens when a “wand” is pushed through the membrane?

12. Passive Transport NO ENERGY required, moves molecules from high concentration to low concentration 1. Osmosis movement of water across a membrane http://schools.moe.edu.sg/chijsjc/Biology/Diffusion&osmosis/osmosis.gif

13. Passive Transport – no energy required 2. Diffusion molecules move from an area of high concentration to low concentration http://iweb.tntech.edu/mcaprio/diffusion-animated.gif

14. Passive Transport – no energy required 3. Facilitated Diffusion movement of molecules from high concentration to low concentration with help of membrane transport proteins Low concentration High concentration http://www.biology.arizona.edu/CELL_BIO/problem_sets/membranes/graphics/CHANNEL.GIF

15. Passive Transport

16. Active Transport Requires energy Moves molecules against concentration gradient Moves from area of low concentration to high concentration Examples: gated channels, sodium/potassium pumps, endocytosis, exocytosis

17. Active Transport Endocytosis and Exocytosis http://media-2.web.britannica.com/eb-media/38/8038-004-A29C9C02.jpg

18. Active Transport Example: Paramecium uses contractile vacuole to regulate water content http://www.cartage.org.lb/en/themes/sciences/BotanicalSciences/MajorDivisions/KingdomProtista/Protists/paramecium.gif

19. Active Transport

20. Modeling Diffusion Lab • Make simulated agar cells in 3x3cm, 2x2cm, and 1x1cm cubes. • Immerse the cells in a “food” solution of sodium hydroxide (NaOH) for 10 minutes. • Pour off NaOH solution, rinse cells. • Cut cells in half. Measure from outside in and record distance NaOH penetrated into cell.

21. Diffusion Lab Questions • Which cell is most efficient in moving materials throughout its entire structure? • What are the limitations to cell size? • How does the surface area relate to the volume of each cell? Surface area = 6 x (side)2 Volume = length x width x height (cm3)

22. Gummy Bear Lab Day 1: Measure L,W,D of bear. Place in a plastic cup and mass the bear and cup. Record in table. Fill ½ full with distilled water. Initial your cup. Place on tray. Write hypothesis of what will happen to bear overnight. Day 2: Pour out water. Measure volume. Mass bear and cup. Record. Fill ½ full with salt water. Write hypothesis of what will happen to bear overnight. Day 3: Pour out water. Measure mass of bear and cup. Write statement of what happened to bear from day 1 to day 3.

23. Solutions Isotonic solution – equal concentrations of solute (salt) inside and outside cell http://www.biologycorner.com/resources/isotonic.gif

24. Solutions Hypotonic solution – less solute in solution, more solute in cell, WATER FOLLOWS SALT, cells swell http://www.biologycorner.com/resources/hypotonic.gif

25. Solutions Hypertonic solution – more solute in solution, less solute in cell, WATER FOLLOWS SALT, cells shrink http://www.biologycorner.com/resources/hypertonic.gif

26. Osmosis in blood cells http://aryatiabdul.files.wordpress.com/2008/07/osmosis2.gif

27. Solutions – how transport affects animals and plants http://kentsimmons.uwinnipeg.ca/cm1504/Image130.gif

28. Transport in Plants Healthy plant cells are crisp due to TURGOR PRESSURE Wilted plant cells are flaccid due to lack of water

29. Transport, again Draw this in your notes Over time, water level changes to make water concentrations equal on both sides of tube TIME Diffusion of water across a semi-permeable membrane = osmosis