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The Effects Of Nitric Oxide On The Development Of Alzheimer's Disease

The Effects Of Nitric Oxide On The Development Of Alzheimer's Disease. Caroline Hsiao. Need. http://www.alz.org/advocacy/2006program/images/charts/5.gif. Need. http://msnbcmedia1.msn.com/i/msnbc/Components/Art/HEALTH/070319/AP_ALZHEIMERS.gif. Plaques. Tangles in Neuron. Plaques.

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The Effects Of Nitric Oxide On The Development Of Alzheimer's Disease

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  1. The Effects Of Nitric Oxide On The Development Of Alzheimer's Disease Caroline Hsiao

  2. Need http://www.alz.org/advocacy/2006program/images/charts/5.gif

  3. Need http://msnbcmedia1.msn.com/i/msnbc/Components/Art/HEALTH/070319/AP_ALZHEIMERS.gif

  4. Plaques Tangles in Neuron Plaques

  5. Alzheimer’s Disease http://pampee.20gig.com/wp-content/uploads/2009/04/alzheimers-disease.jpg http://www.alzheimer.ca/english/disease/whatisit-intro.htm

  6. Alzheimer’s Disease http://www.topnews.in/health/files/alzheimers-brain.jpg

  7. Nitric Oxide As A Free Radical http://www.inchem.org/documents/jecfa/jecmono/40abcj11.gif http://www.inchem.org/documents/jecfa/jecmono/40abcj11.gif

  8. Oxidative Stress • Cigarette smoke is used as an example shown here http://healthy-lifestyle.most-effective-solution.com/wp-content/uploads/2007/10/oxidative-stress.jpg

  9. Age-Dependent Neurodegeneration and Alzheimer-Amyloid Plaque Formation in Transgenic Drosophila Greeve, and Kretzschmar,

  10. Dissecting the pathological effects of human A40 and A42 in Drosophila: A potential model for Alzheimer’s disease By: Iijima and Liu

  11. Investigation of Free Radicals in Cigarette Mainstream Smoke M. Ghoshand P. Ionita

  12. Purpose The purpose of this experiment was to find if nitric oxide have any effect on the development of Alzheimer's disease. Alternate: Nitric Oxide will cause an increase in the development of Alzheimer's symptoms in drosophila for all groups tested. Null Hypothesis: there will be no change in any of these groups at all.

  13. Experimental Design Protocols Used: GrittaTettweiler, Mathieu Miron, Mark Jenkins, 2005 ELIZABETH J. HARRY, KIT POGLIANO, AND RICHARD LOSICK 1995

  14. The Effects of Nicotine and Nitric Oxide on the development of Amyloid beta plaques in wild type and APP drosophila Wild Type – no nitric oxide n = 20 APP Drosophila–no nitric oxide n= 20 • APP Drosophila –nitric oxide for 1 minute n= 20 (6 days old) • APP Drosophila –nitric oxide for 5minute n= 20 (6 days old) Wild Type–nitric oxide for 1 minute n= 20 Wild Type–nitric oxide for 1 minute n= 20 Wild Type-nitric oxide n= 20 (6 days old) 5 trials will be run, each for approximately 12-13 days per trial. After all slides have been made they will be observed using the fluorescent microscope and the images will be taken using a digital camera and then using Photoshop the total amount of amyloid beta plaques will be measured. Using SPSS statistical analysis will be done using an ANOVA followed by a Scheffe Posthoc test with p< .05

  15. Budget

  16. Do Ability • All materials are orderable from school approved companies • Drosophila can be kept alive with relative ease in school lab • School lab already has a microscope necessary for observation

  17. Bibliography • Crag A. Michelli, William P Esler and W. Taylor Kimberley, Y-Secretase/presenilin inhibitors for Alzheimer’s disease phenocopyNotch mutations in Drosophila, FASEB, 3/09/2003, volume 17, pgs 79-81 • Crowther DC, Kinghorn KJ, Miranda E, Page R, Curry JA, et al. (2005) IntraneuronalAb, non-amyloid aggregates and neurodegeneration in a Drosophila model of Alzheimer’s disease. Neuroscience 132: 123–135. • Harry EJ, Pogliano K, Losick R (1995) Use of immunofluorescence to visualize cellspecific gene expression during sporulation in Bacillus subtilis. J Bacteriol • 177, volume 12, pgs 3386-93. • GrittaTettweiler, Mathieu Miron, Mark Jenkins, Nahum Sonenberg, and Paul F. Lasko, Starvation and oxidative stress resistance in Drosophila are mediated through the eIF4E-binding protein, d4E-BP, Genes Dev, August 15 2005, volume 19, pgs 1840–1843. • IsabellGreeve, Doris Kretzschmar, Jakob-Andreas Tscha¨pe, AnikaBeyn, Claire Brellinger, Michaela Schweizer, Roger M. Nitsch, and Rita Reifegerste1, Age-Dependent neurodegeneration and Alzheimer-Amyloid Plaque Formation in Transgenic Drosophila, The Journal of Neuroscience, April 21, 2004, volume 16, pgs 3899 –3906 • Koichi Iijima, Hsin-Ping Liu, Ann-Shyn Chiang, Stephen A. Hearn, Mary Konsolaki, Yi ZhongDissecting the pathological effects of human Aβ40 and Aβ42 in Drosophila: A potential model for Alzheimer's disease, PNAS, October 31, 2003, volume 101, pgs 6623–6628 • MUHAMMAD ARSHAD, MARIE PAULSSON, and PETR DEJMEK, Rheology of Buildup, of Acid Casein Gels Breakdown, and Rebodying, March 3 1993, J Dairy Sci volume 76, pgs 331-3316 • P. Mpountoukas, A. Vantarakis, E. Sivridis and T. Lialiaria, Cytogenetic study in cultured human lymphocytes treated with three commonly used preservatives, January 30 2008, Food and Chemical Toxicology, volume 46, pgs 2390-2393 • Pogliano K, Harry E, Losick R (1995) Visualization of the subcellular location of • sporulation proteins in Bacillus subtilis using immunofluorescence microscopy. Mol • Microbiol, volume 18, pgs 459-70 • Yihong Ye and Mark E. Fortini Apoptotic Activities of Wild-type and Alzheimer’s Disease-related Mutant Presenilins in Drosophila melanogaster, The Journal of Cell Biology, September 20 1999, Volume 146, pgs 1351–1364

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