Electrophoresis

1. Electrophoresis By John Darwin, Wes Gallagher, and Thompson Grant

2. History of Electrophoresis • Generally known as the electrokinetic phenomenon • It was discovered in Russia by a man named Reuss in 1809 • It is the motion of dispersed particles relative to a fluid under the influence of an electric field that is space uniform

3. Electrophoresis in Forensic Science • Gel Electrophoresis for: • 1.Map the order of restriction fragments within chromosomes • 2.To analyze DNA variation within a population by restriction fragment length polymorphisms • 3. To determine the nucleotide sequence of a piece of DNA

4. Process • The gel is made and then the molecules are moved through the gel by the electromotive force. • After the molecules have been moved through the gel, the gel is stained to make the molecules visible. • Ethidium Bromide is used to stain the molecules • The molecules form parallel lines and separate from each other and the size shows the molecule’s weight

5. Applications • Used in forensics, molecular biology, genetics, microbiology, and biochemistry • Results can be seen with UV light and a gel imaging device • The results can give DNA identification

6. DNA Electrophoresis • The identification is used by the different proteins and nucleic acids separating by size • The results are shown on a device called an electropherogram

7. Sources • http://en.wikipedia.org/wiki/Gel_electrophoresis • http://en.wikipedia.org/wiki/Electrophoresis • http://en.wikipedia.org/wiki/DNA_electrophoresis • http://learn.genetics.utah.edu/units/biotech/gel/