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Microscope & Cells

BIOL 100L. Microscope & Cells. Microscope, Cells & Mitosis Lab. Prelab Quiz Models: cells Prepared slides in order Crossed threads- just focus Letter “e”- draw at 4, 10, 40x mag 6 slides- draw at best mag include sperm and blood at 100x. Wet Mount slides Pond water Yeast Cheek cell.

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Microscope & Cells

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  1. BIOL 100L Microscope & Cells

  2. Microscope, Cells & Mitosis Lab • Prelab Quiz • Models: cells • Prepared slides in order • Crossed threads- just focus • Letter “e”- draw at 4, 10, 40x mag • 6 slides- draw at best mag • include sperm and blood at 100x • Wet Mount slides • Pond water • Yeast • Cheek cell

  3. Carrying a Microscope

  4. Storing The Microscope • Return the lowest power objective in place • Wrap the cord around the base • Return dustcover

  5. Cleaning the Microscope • Use lens paper on all glass parts of the microscope. • Clean oil immersion lens with chemicals provided by your instructor

  6. Parts of the Microscope • Ocular • Body tube • Nosepiece • Objective lenses (10, 40, 100x) • Arm • Course adjustment knob • Fine adjustment knob • Stage • Stage clips • Aperture • Diaphragm • Light • Base

  7. Microscope Parts

  8. Using the microscope • Always observe using the LOWEST POWER • Focus using the COARSE ADJUSTMENT KNOB to bring the object into focus. Then use FINE ADJUSTMENT KNOB . • Focus, and then move to a higher power objective, if needed. • Use only the FINE ADJUSTMENT KNOB when using the HIGHEST (longest) POWER OBJECTIVE. • Keep both eyes open to reduce eyestrain. • Determine total magnification of the object by multiplying the power of the ocular (10x) the power by the power of the objective.

  9. Preparing a slide

  10. Oil Immersion Lens When finished: clean objective and prepared slide with lens paper

  11. Animal Cell

  12. Plant Cell

  13. Chose a total of 6 prepared slides and draw at best magnification Use oil for sperm and blood

  14. 4x 10x 100x

  15. Some slides may have more than one tissue type Adipose Hyaline cartilage

  16. Pseudostratified ciliated columnar epithelium

  17. Blood (RBC & WBC) use oil

  18. sperm use oil

  19. Cheek Cell 0.9% saline

  20. Cheek Cell • Scrape inside cheek • Swirl toothpick with cells in 0.9% saline • Place a small drop 0.01% methylene blue on saline saline

  21. Cheek Cell • Place coverslip • Observe under microscope • Draw what you see • Clean up

  22. Cheek Cell

  23. Dispose Sharps

  24. Clean Up

  25. Microscope Parts Quiz

  26. Microscope Quiz • Which objective uses oil? • If your ocular is 10x and your objective is 40x what is the total magnification of your image? • Why do you need a cover slip and how do you avoid an air bubble? • Where do you dispose slides?

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