1 / 6

Effects of Tetracycline and Kanamycin on Pseudomonas fluorescens

Effects of Tetracycline and Kanamycin on Pseudomonas fluorescens. Naomi Drake, Mary Powell, Alexis County. Low Down. Question: Which antibiotic, Tetracycline or Kanamycin, has the greatest effect on the lag phase of Pseudomonas fluorescens ?

darin
Download Presentation

Effects of Tetracycline and Kanamycin on Pseudomonas fluorescens

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Effectsof Tetracycline and Kanamycin on Pseudomonas fluorescens Naomi Drake, Mary Powell, Alexis County

  2. Low Down • Question: Which antibiotic, Tetracycline or Kanamycin, has the greatest effect on the lag phase of Pseudomonas fluorescens? • Hypothesis: The organism’s lag phase would be more significantly extended in the presence of kanamycin as opposed tetracycline.

  3. Initial growth: Dilution 1:10,000- reached .4 OD @ 9:45:05 hours [.392, .443 @ 10:00:05 hours] Extended Lag phase: Dilution 1:1000- reached .4 OD @20:00:05 hours [.394, .422 @ 20:15:05 hours] Note: Dilutions 1:10 and 1:100 fully inhibited growth

  4. Initial growth: Control (no antibiotic)- reached 0.4 OD @ 4:30:05 hours [.397 OD, .424 OD @ 4:45:05 hours] Extended Lag Phase: Dilution 1:1600 – reached 0.4 OD @ 28:30:05 hours [.398 OD, .412 OD @28:45:05 hours]

  5. Tetracycline vsKanamycin

  6. Conclusions • Based on the data collected, P. fluorescens is resistant to tetracycline in various dilutions ranging from 1:10 to 1:12,800. • In the presence of Kanamycin however, the bacteria was susceptible, in varying ratios, ranging from dilutions 1:2 to 1:12,800.

More Related