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Effects of Glucose on the Growth Curve of Pseudomonas fluorescens

Effects of Glucose on the Growth Curve of Pseudomonas fluorescens. Muhammad Khan & Andrew Passer November 28, 2011. Our Question:. What effect does the addition of glucose have on the growth curve of Pseudomonas fluorescens ?. Interest in Glucose. Experiment 2. Experiment 3. ?. ?.

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Effects of Glucose on the Growth Curve of Pseudomonas fluorescens

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  1. Effects of Glucose on the Growth Curve of Pseudomonas fluorescens Muhammad Khan & Andrew Passer November 28, 2011

  2. Our Question: What effect does the addition of glucose have on the growth curve of Pseudomonas fluorescens?

  3. Interest in Glucose Experiment 2 Experiment 3 ? ?

  4. Experiment 4 Supports Experiment 3

  5. Is glucose metabolism in P. fluorescens acquired or inherited? We could not “shock” P. fluorescens into increased growth by raising it in a glucose-rich, tryptone-poor environment prior to inoculation.

  6. Calculating "Growth Points"

  7. What Concentration of Glucose Gives the Highest Level of Growth? Average: 3.418% glucose

  8. Conclusion • Addition of glucose has a positive effect on the growth of P. fluorescens. • Glucose metabolism is an inherited trait for this species. • Optimum growth occurs at about 3.4% glucose.

  9. Discussion & Future Directions • More time needed for P. fluorescens to grow. • Is the prolonged increase in OD actual growth? • What are the waste products of the different metabolic pathways (amino acid vs. sugar)? • How do the waste products affect the stationary phase?

  10. Any questions?

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