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Hypercholesterolemia

Hypercholesterolemia. Pick up sample Get new tip Set to 30 m l Push plunger to 1 st stop Lower tip to bottom of tube Release plunger. Drop off sample Lower tip into well Push plunger to 2 nd stop Raise tip out of well Release plunger Discard tip. Load tray into chamber

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Hypercholesterolemia

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  1. Hypercholesterolemia

  2. Pick up sample Get new tip Set to 30 ml Push plunger to 1st stop Lower tip to bottom of tube Release plunger Drop off sample Lower tip into well Push plunger to 2nd stop Raise tip out of well Release plunger Discard tip Load tray into chamber • Wells to negative end • Fill buffer to a level above the gel • Double stack A B C D E F

  3. Hypercholesterolemia • How do our cells use cholesterol? • Why does the liver send out cholesterol in LDLs (initially as VLDLs) if HDLs just bring them back? • What is the problem with having too much LDL? • What method(s) do we have for combatting a high LDL/HDL ratio? • A mutated allele for a certain gene leads to FH. For what does the normal allele code?

  4. Hypercholesterolemia • What are the genotypes of Patients 1, 2, and 3? • Explain two possible reasons for the band locations on gels… 1st: 4 and 5 as compared to 1-3 and 6. 5th: 7-12 as compared to 1-3. • Explain how the following changes in procedure would affect band locations: • Doubling the agar powder when making gels. • Mixing up the connections to the power supply.

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