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This study delves into genome size variations, maternal factors, and gene expression patterns in T. miscellus, comparing species like T. dubius and T. pratensis. Fluorescent labeling techniques reveal key genomic insights.
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Genome size and change Genome composition and arrangement Maternal factors in T. miscellus
Averages 11.13 11.70 12.84 24.20 21.02
1: 2.3 1: 4.8 1: 12 1: 1.1 1: 1.3 1: 2.1 1.6 :1 1: 6.7 1: 1.2 1.1 :1
DAPI stained TGP7 (red) and TRS (green)-labeled TPRMBO-labeled TPRMBO-labeled TGP7-labeled DAPI stained D+E+F T. dubius T. pratensis T. porrifolius DAPI stained H+TGP7-labeled Just DAPI??? TGP7 and TPRMBO
5S (green, if you squint just right) 18S-5.8S-26S (yellow) T. dubius T. pratensis T. porrifolius
T. miscellus T. mirus DAPI TPRMBO and TPG7 TPRMBO and TGP7 DAPI
D1 : 5S from T. dubius P1, P6 : 5S from T. porrifolius P4 : 18S-5.8S-26S from T. porrifolius 5S (green, if you squint just right) 18S-5.8S-26S (yellow or red) T. Miscellus T. mirus T. mirus
Gene expression in T. miscellus. Tm Tm’ Tp Td cDNA-AFLP Monomrphic Paternal expression
Gene expression in T. miscellus. Paternal expression Gene silencing in the polyploids Coexpression of homeologous loci Tm Tm’ Tp Td
Gene expression in T. miscellus. Tm Tm’ Tp Td Coexpression of homeologous loci Coexpression of homeologous loci Maternal expression