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Biotechnology

Chapter 20. Biotechnology. Questions prepared by Christopher Gregg Louisiana State University Louise Paquin McDaniel College. Which of the following is an example of "recombinant DNA technology?". combining alternate alleles of a gene in a single cell

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Biotechnology

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  1. Chapter 20 Biotechnology Questions prepared by Christopher GreggLouisiana State University Louise PaquinMcDaniel College

  2. Which of the following is an example of "recombinant DNA technology?" • combining alternate alleles of a gene in a single cell • manipulating a meiotic crossing-over event • cloning genes from homologous pairs of chromosomes • introducing a human gene into a bacterial plasmid • alternate alleles assorting independently

  3. How does a bacterial cell protect its own DNA from restriction enzymes? • a) by forming "sticky ends" of bacterial DNA to prevent the enzyme from attaching • b) by using DNA ligase to seal the bacterial DNA into a closed circle • c) by reinforcing the bacterial DNA structure with covalent phosphodiester bonds • d) by adding histones to protect the double-stranded DNA • e) by adding methyl groups to adenines and cytosines

  4. One strand of a DNA molecule has the sequence3′-GGATGCCCTAGGCTTGTT-5′Which of the following is the complementary strand? • 3′-AACAAGCCTAGGGCATCC-5′ • 3′-CCTACGGGATCCGAACAA-5′ • 5′-AACAAGCCTAGGGCATCC-3′ • 5′-GGATGCCCTAGGCTTGTT-3′

  5. Which of the following DNA molecules is most likely to be cut by a restriction enzyme? • 5′-AAGCCT-3′ • 5′-GGAAGG-3′ • 5′-GGATCC-3′ • 5′-AATTAA-3′

  6. You have a restriction enzyme that makes a blunt cut between an A and a T. What will the sizeof the DNA fragments be after the following DNA molecule is cut with this restriction enzyme:5′-TTGTTCGGATCCCGTAGG-3′? • one 9-bp fragment, one 6-bp fragment, and one 3-bp fragment • one 15-bp fragment and one 3-bp fragment • one 18-bp fragment • two 9-bp fragments

  7. You have isolated this eukaryotic gene and wish to express the protein it codes for in a culture of recombinant bacteria. Will you be able to produce a functioning protein with the gene as is? • yes • No, the exons will need to be cut out and the introns spliced back together. • No, the introns will need to be cut out and the exons spliced back together. • No, the exons will need to be cut out, the introns translated individually, and the peptides bound together after translation.

  8. This segment of DNA is cut at restriction sites 1 and 2, which creates restriction fragments A, B, and C. Which of the following electrophoretic gels represents the separation of these fragments? • Answer a • Answer

  9. A dideoxy chain-terminating sequencing reaction produces the following batch of labeled DNA strands. What is the sequence of the template DNA strand? • 5′-AGGCTT-3′ • 5′-AAGCCT-3′ • 5′-TCCGAA-3′ • 5′-TTCGGA-3′

  10. The photograph shows Rainbow and CC (CC is Rainbow’s clone). Why is CC’s coat pattern different from Rainbow’s given that CC is genetically identical? • X chromosome inactivation • heterozygous at coat color gene locus • environmental effects on gene expression • all of the above

  11. The first cell whose entire genome was sequenced was which of the following? • a) H. influenzae in 1995 • b) H. sapiens in 2001 • Rice in 1955 • Tobacco mosaic virus • HIV in 1998

  12. For discussion: • Why has gene therapy had only rather limited success?

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