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How Cells Divide

How Cells Divide. Chapter 10. Bacterial Cell Division. Bacteria divide by binary fission . -the single, circular bacterial chromosome is replicated -replication begins at the origin of replication and proceeds bidirectionally -new chromosomes are partitioned to opposite ends of the cell

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How Cells Divide

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  1. How Cells Divide Chapter 10

  2. Bacterial Cell Division Bacteria divide by binary fission. -the single, circular bacterial chromosome is replicated -replication begins at the origin of replication and proceeds bidirectionally -new chromosomes are partitioned to opposite ends of the cell -a septum forms to divide the cell into 2 cells

  3. Eukaryotic Chromosomes Eukaryotic chromosomes– -linear chromsomes -every species has a different number of chromosomes -composed of chromatin– a complex of DNA and proteins -heterochromatin– not expressed -euchromatin –expressed regions

  4. Eukaryotic Chromosomes Chromosomes are very long and must be condensed to fit within the nucleus. -nucleosome – DNA wrapped around a core of 8 histone proteins -nucleosomes are spaced 200 nucleotides apart along the DNA -further coiling creates the 30-nm fiber or solenoid

  5. Eukaryotic Chromosomes The solenoid is further compacted: -radial loops are held in place by scaffold proteins -scaffold of proteins is aided by a complex of proteins called condensin karyotype: the particular array of chromosomes of an organism

  6. Eukaryotic Chromosomes Chromosomes must be replicated before cell division. -Replicated chromsomes are connected to each other at their kinetochores -cohesin – complex of proteins holding replicated chromosomes together -sister chromatids: 2 copies of the chromosome within the replicated chromosome

  7. Eukaryotic Cell Cycle The eukaryotic cell cycle has 5 main phases: 1. G1 (gap phase 1) 2. S (synthesis) 3. G2 (gap phase 2) 4. M (mitosis) 5. C (cytokinesis) The length of a complete cell cycle varies greatly among cell types. interphase

  8. Interphase Interphase is composed of: G1 (gap phase 1) – time of cell growth S phase – synthesis of DNA (DNA replication) - 2 sister chromatids are produced G2 (gap phase 2) – chromosomes condense

  9. Interphase Following S phase, the sister chromatids appear to share a centromere. In fact, the centromere has been replicated but the 2 centromeres are held together by cohesin proteins. Proteins of the kinetochore are attached to the centromere. Microtubules attach to the kinetochore.

  10. Interphase During G2 the chromosomes undergo condensation, becoming tightly coiled. Centrioles (microtubule-organizing centers) replicate and one centriole moves to each pole.

  11. Mitosis Mitosis is divided into 5 phases: 1. prophase 2. prometaphase 3. metaphase 4. anaphase 5. telophase

  12. Mitosis Prophase: -chromosomes continue to condense -centrioles move to each pole of the cell -spindle apparatus is assembled -nuclear envelope dissolves

  13. Mitosis Prometaphase: -chromosomes become attached to the spindle apparatus by their kinetochores -a second set of microtubules is formed from the poles to each kinetochore -microtubules begin to pull each chromosome toward the center of the cell

  14. Mitosis Metaphase: -microtubules pull the chromosomes to align them at the center of the cell -metaphase plate: imaginary plane through the center of the cell where the chromosomes align

  15. Mitosis Anaphase: -removal of cohesin proteins causes the centromeres to separate -microtubules pull sister chromatids toward the poles -in anaphase A the kinetochores are pulled apart -in anaphase B the poles move apart

  16. Mitosis Telophase: -spindle apparatus disassembles -nuclear envelope forms around each set of sister chromatids -chromosomes begin to uncoil -nucleolus reappears in each new nucleus

  17. Cytokinesis Cytokinesis – cleavage of the cell into equal halves -in animal cells – constriction of actin filaments produces a cleavage furrow -in plant cells – plasma membrane forms a cell plate between the nuclei -in fungi and some protists – mitosis occurs within the nucleus; division of the nucleus occurs with cytokinesis

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