1 / 8

HIV-1/HIV-2 COMBINATION SCREENING AND OUTCOMES OF FOLLOW UP William R. Oleszko, Ph.D.

HIV-1/HIV-2 COMBINATION SCREENING AND OUTCOMES OF FOLLOW UP William R. Oleszko, Ph.D. Associate Director, Public Health Laboratory Department of Health and Mental Hygiene New York City. HIV ANTIBODY TESTING – 2004 April 2004: Initiated use of new HIV antibody screening assay

declan
Download Presentation

HIV-1/HIV-2 COMBINATION SCREENING AND OUTCOMES OF FOLLOW UP William R. Oleszko, Ph.D.

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. HIV-1/HIV-2 COMBINATION SCREENING AND OUTCOMES OF FOLLOW UP William R. Oleszko, Ph.D. Associate Director, Public Health Laboratory Department of Health and Mental Hygiene New York City

  2. HIV ANTIBODY TESTING – 2004 April 2004: Initiated use of new HIV antibody screening assay •BioRad’s HIV-1/HIV-2 Plus “O” EIA April – December 2004: 73,955 specimen submissions 96.5% NEGATIVE for antibody to HIV-1/HIV-2 3.3% POSITIVE for antibody to HIV-1 0.2% INCONCLUSIVE for antibody to HIV-1

  3. HIV-2 ANTIBODY

  4. CATEGORIES FOR SPECIAL STUDY • Atypical but reactive HIV-1 Western blot banding pattern • Inconclusive: pregnant or two HIV-1 inconclusives within one month • Area of birth = Africa/Asia • HIV-2 reactivity • AIDS symptoms and HIV antibody negative • Child of HIV positive mother • HIV Positive, no ART, undetectable viral load, declining CD4 • HIV positive, on ART, undetectable viral load, declining CD4 • OraQuick preliminary positive confirmatory result = inconclusive

  5. ASSAYS PERFORMED ON SPECIAL STUDY SPECIMENS Current: • HIV-1/HIV-2 Multispot Rapid EIA • HIV V3 serotype • CD4/CD8 T-cell Enumeration • HIV-1 DNA PCR • HIV-2 DNA PCR • Viral load - HIV-1 RNA PCR & HIV-1 bDNA Future: • HIV Genotyping/Resistance Testing

  6. SEEK AND YE SHALL FIND!

  7. Public Health Laboratory PHL: Sara T. Beatrice, Ph.D., Assistant Commissioner Retrovirology Laboratory: Amado Punsalang, Jr., Ph.D. Robert Pirillo – Community Coordinator Mona El-Fishawy - HIV Screening Lab Mohammad Younis – HIV Supplemental Testing Lab Ed Lee. Ph.D. – HIV PCR Lab Orlin Trochev, MD, Ph.D. – HIV Genotyping/Resistance Lab

More Related