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Carbon Tracing from Glucose. Glucose radiolabeled at specific Carbons Can determine fate of individual carbons Carbons 1,6 1 st cycle: 2,3 of oxaloacetate Starting at 3 rd cycle ½ radioactivity CO 2 /cycle Carbons 2,5 1 st cycle: 1,4 of oxaloacetate
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Carbon Tracing from Glucose • Glucose radiolabeled at specific Carbons • Can determine fate of individual carbons • Carbons 1,6 • 1st cycle: 2,3 of oxaloacetate • Starting at 3rd cycle ½ radioactivity CO2/cycle • Carbons 2,5 • 1st cycle: 1,4 of oxaloacetate • 2nd cycle: all eliminated as CO2 • Carbons 3,4 • All eliminated at CO2 during Pyruvate Acetyl-CoA
Movie of pyruvate dehydrogenase (and others) can be found here: http://www.rpi.edu/dept/bcbp/molbiochem/MBWeb/mb1/part2/krebs.htm