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Cellular respiration. The process of recharging ATP Textbook pp 95-100. You think… . Is ‘Respiration’ the same as ‘Breathing’?? No. Respiration is…. The release of energy from food. ATP. ATP is an energy carrier within the cell
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Cellular respiration The process of recharging ATP Textbook pp 95-100
You think… • Is ‘Respiration’ the same as ‘Breathing’?? • No. Respiration is…. The release of energy from food
ATP • ATP is an energy carrier within the cell • ATP can’t be stored nor can it be exported, so it must be used as it is produced
The Regeneration of ATP Cellular respiration is the process that recharges ATP Go to TB p 95 • A living organism uses ATP continuously, but ATP is a renewable resource that can be regenerated by the addition of phosphate to ADP. The energy for this comes from the breakdown of food molecules.
Other Carriers • Go to p 96
The need for energy • Think of 5 reasons a human body, including all its cells, needs energy • creation (such as protein and polysaccharide manufacture) • active transport (ion pumps, cytosis) • movement (microtubules, actin and myosin filaments, cilia) • generate heat to maintain body temperature
Cellular respiration • Look up the equation for cellular respiration. • Complete Worksheet
Cellular respiration • The complete oxidation of glucose involves over 20 different reactions, each catalysed by a different enzyme. Respiration does not break down glucose in a single step. Instead, glucose is broken down gradually releasing about 35% of the available energy to produce ATP. The rest is lost as heat energy
Overview of respiration 3 major processes Glycolysis The Krebs Cycle Electron Transport
Use your Text Books to match the steps to their major functions and correct order The breakdown of Glucose to Pyruvate Occurs on the cytoplasm NAD carriers get filled with Hydrogen to form NADH Occurs in the inner matrix of the mitochondria The stage where most ATP are charged up Electrons are passed over cytochromes in the on the membranes of the mitochondria Step 1. 2. 3 Electron Transport Glycolysis The Krebs Cycle
CELLULAR RESPIRATION 2Learning Intentions: Understand GLYCOLYSIS Understand Formation of Acetyl CoA
Glycolysis Taking a 6 Carbon Glucose and breaking it into two 2 Carbon molecules.
Process 1: Glycolysis Net yield is: 2ATP, 2NADH & 2 pyruvate
Link reaction between Glycolysis and The Krebs Cycle: occurs in the mitochondrial matrix The pyruvic acid in Glycolysis loses a Carbon (through the creation of a CO2) It is now a 2 Carbon compound called acetyl Acetyl is carried by Coenzyme A – together they are Acetyl CoA Acetyl CoA moves to the Krebs Cycle.
CELLULAR RESPIRATION 3Learning Intentions: Identify Mitochondrial structureUnderstand Electron Transport
First - Mitochondria • Use TB p 81 to label this
Krebs cycle (citric acid cycle): takes place in the mitochondrial matrix Acetyl CoA Animation & another
Electron transport chain: takes place in and across the cristae • The electron transport chain consists of a sequence of protein carriers in the membrane of the cristae • Electrons and protons are accepted by oxygen as follows: O2 + 4H+ + 4e- 2H2O • The process illustrated is defined as oxidative phosphorylation(the manufacture of ATP in the presence of oxygen)
Electron & H+ transport coupled with ATP synthesis animation
ATP “balance sheet” for respiration of glucose Cellular respiration song
CELLULAR RESPIRATION 4Learning Intentions: Distinguishing between aerobic and anaerobic respiration
Other substrates for respiration • Starch • Glycogen • Triglycerides (fatty acids are broken down into 2C molecules and enter the Krebs cycle as acetyl CoA; glycerol enters glycolysis as a triose phosphate) • Proteins (very rarely: amino acids are deaminated and converted into pyruvate, acetyl CoA or Krebs cycle intermediates)
Aerobic respiration • When oxygen is present, pyruvate follows a certain pathway: • It enters the mitochondria and undergoes a link reaction • The product of the link reaction enters the Krebs cycle (or Citric acid cycle) • And finally, H atoms (H+ and e-), that have been removed during the steps above, enter the electron transport chain
Anaerobic respiration • Occurs in the cytoplasm • Does not require oxygen but does occur in its presence • Still involves Glycolysis, but does not follow through the Acetyl CoA Link reaction • Generates a very small amount of ATP • Produces poisonous bi-products
Fate of pyruvate when there is no oxygen • Alcoholic fermentation in yeast cells • Lactic acid fermentation in muscle cells • Lactic acid is transported to the liver and oxidised to pyruvate & then to glucose. The oxygen needed for this is called the ‘oxygen debt’ of anaerobic respiration