Needs version 1.1.3.8A

1. Needs version 1.1.3.8A

2. Step 1: Check Settings • „Enable SampleTrack“ is unchecked • „Manually destination selection“ is checked • „Clean ... “ is unchecked

3. Open procedure editor dialog Two main procedures will be defined: 1. Fill syringe with the total solvent amount 1200µl for 12 tubes. Name: „Load Syringe DMSO 1200.gsp“ 2. Dispense partial solvent amount 100µl into one destination. Name: „Dispense Volume 100.gsp “ (with sub procedure „Sub Disp Volume 100.gsp “)

4. Step 2: Edit procedure „Load Syringe DMSO 1200.gsp“ 1. Check the file name„Dispense Volume 100.gsp “ 2. Select Source Type: „Solvent in Tray“. • 3. Select your own values for: • - Solvent name • Volume (Speed ...) • Hint: Never forget „Update“ and „Save“ after modifications.

5. Step 3a: Edit dispense sub procedure „Sub Disp Volume 100.gsp “ Press the button „Edit“ to edit or create a procedure of the type „Dispense“.

6. Step 3a: Edit dispense sub procedure „Sub Disp Volume 100.gsp “ Check the file name „Sub Disp Volume 100.gsp“. • Select your own values for: • Volume • Z offset height (Speed ...).

7. Step 3b: Edit dispense main procedure „Dispense Volume 100.gsp “ Check the file name „Dispense Volume 100.gsp “ Select the Source Type: „Gas Gap“ Select your Dispense procedure. Volume = 0

8. Step 4: Start Automation to fill sample loop • Select procedure • „Load Syringe DMSO 1200.gsp“. Start Automation. Hint: Solvent not used in procedure! 2. Select one dummy source and destination position (not used inside procedure!). 3. Create Order and continue with „OK“.

9. Step 5: Create Orders to fill rack matrix. Open Dialog „Create new Orders“. Do NOT start automation! Syringe would be homed zero. Procedure finished OK: solvent filled in Sample loop.

10. Step 6: Create Orders to fill rack matrix • Select procedure • „Dispense Volume 100.gsp“. Hint: Solvent not used in procedure! 2. Select 12 source and destination positions (destinations will be used). 3. Create Orders and close dialog with „OK“.

11. Step 7: Start preparation to fill rack matrix Do NOT start automation! Syringe would be homed to zero. Use menu „Service Tools“. Sub menu: „Prepare all Orders“.

12. Step 7: Running preparation