Ma-Mel-48a Ma-Mel-48b Ma-Mel-48c

1. Ma-Mel-48a Ma-Mel-48b Ma-Mel-48c + IFN-, sec mAb only w/o IFN-, sec mAb only + IFN- w/o IFN- HLA-DR HLA Class I HLA-DR (L243) HLA class I (W6/32) Supplementary Fig. S2:The different Ma-Mel-48 cell lines were treated with IFN- (500 U/ml) for 48 h, controls were left untreated. Cells were stained with mAb L243 and mAb W6/32 for detection of HLA-DR and HLA class I antigen expression, respectively. Representative histograms from one of three independent experiments are depicted.