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Humoral Immunity presented by

Humoral Immunity presented by. Dr. MAHROO MIRAHMADIAN Professor of Immunology Tehran University of Medical Sciences. Antibodies. Antibody Structure and function. Protein electrophoresis. Multiple Myeloma. Antigen Binding Site. Antibodies are Proteins that Recognize Specific Antigens.

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Humoral Immunity presented by

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  1. Humoral Immunitypresented by Dr. MAHROO MIRAHMADIAN Professor of Immunology Tehran University of Medical Sciences

  2. Antibodies

  3. Antibody Structure and function

  4. Protein electrophoresis

  5. Multiple Myeloma

  6. Antigen Binding Site

  7. Antibodies are Proteins that Recognize Specific Antigens

  8. Hypervariable regions

  9. Antibody is a flexible molecule

  10. Immunoglobulin Domain

  11. Ig Superfamily

  12. Proteolytic Digestion A MW of 150,000 Da A MW of 100,000 Da A MW of 25,000 Da A MW of 45,000 Da A MW of 50,000 Da A MW of 50,000 Da

  13. Proteolytic Digestion

  14. Antibody classes

  15. IgG subclasses

  16. Opsonization

  17. Complement activation

  18. Neutralization

  19. ADCC

  20. Effector functions of IgG Antibody • Opsonization of bacteria for phagocytosis by macrophages and neutrophils • Neutralization of toxins • Activation of the classical pathway of complement • Antibody dependent cell mediated cytotoxicity (ADCC) by NK cells. • Neutralization of microbes • Transfer of maternal antibody across the placenta and gut.

  21. Effector functions of IgG subclasses • IgG1, IgG3 and IgG4 readily cross the placenta (neonatal immunity) • IgG3, IgG1 are effective complement activator, and IgG4 is not able to activate complement. • IgG1 and IgG3 bind to Fc receptors on phagocytic cells and thus mediate opsonization.

  22. sIgA

  23. Neutralization

  24. Effector functions of IgA antibody • Mucosal immunity: secretion of IgA into the lumens of the gastrointestinal and respiratory tract. • Neonatal immunity: transfer of maternal IgA from breast milk • Neutralization of bacteria, viruses and toxins

  25. IgM

  26. Effector functions of IgM antibody • Elevated level of IgM usually indicated either recent infection or recent exposure to the antigen. • Isohemagglutinins. • Antigen receptor of naive B lymphocyte. • Activation of classical pathway of complement. • IgM is the first antibody produced in a primary response to an antigen.

  27. Effector functions of IgD antibody IgD, together with IgM, is expressed by matureB cell

  28. IgE

  29. Allergen cross-linkage of receptor-bound IgE on mast cells induces degranulation

  30. Mast cells basophils and activated eosinophils bind IgE antibody via Fc eR • Mast cells contain cytoplasmic granules that contain mixture of chemical mediators: histamine which make local blood vessels permeable. • Lipid mediators such as prostaglandin D2 and leukotriene C4 and to secret cytokines such as TNF-a.

  31. ADCC

  32. Effector functions of IgE antibody • IgE antibody mediate immediate hypersensitivity reaction. • Antibody dependent cell mediated cytotoxicity (ADCC) involving eosinophils

  33. Isotype • Allotype • Idiotype

  34. Idiotype

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