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Preparation of pooled plasma as a control for coagulation tests

Preparation of pooled plasma as a control for coagulation tests. Preparation of pooled plasma. Best for large hospitals which have a busy blood bank (with a facility of -80 o C freezer). Preparation of pooled plasma.

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Preparation of pooled plasma as a control for coagulation tests

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  1. Preparation of pooled plasma as a control for coagulation tests

  2. Preparation of pooled plasma • Best for large hospitals which have a busy blood bank (with a facility of -80oC freezer)

  3. Preparation of pooled plasma • Healthy donor’s plasma segments are collected and immediately frozen at -80º C. • All these segments are thawed at 37º C, cut and pooled in a plastic container. • This pooled plasma is centrifuged at 4000 rpm for 10 minutes to obtain platelet poor plasma. • Platelet count is analyzed for representative pool. Platelet count should be <10000 ×109L.

  4. Pooled plasma – Normal control • This pooled plasma is aliquoted in vials and immediately stored at -80º C. • Pooled plasma which has been prepared is analysed on ACL analyzer for PT, PTT & PT derived Fibrinogen. • This pooled plasma is used as a Normal control with every batch of samples.

  5. Pooled plasma -Abnormal control • Pooled plasma diluted with phosphate buffer saline (pH-7.2 )(1:3) • This may be used as an abnormal control.

  6. PT, PTT & derived Fibrinogen readings of control are monitored for every batch. The mean of 21 readings are taken and it is programmed in reference data of ACL analyzer for PT, PTT & Fibrinogen.

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