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RFLP

RFLP. “Restriction Fragment Length Polymorphism” Basic idea: If two DNA sequences have different numbers or locations of restriction sites, they will differ in their restriction enzyme digest pattern Uses: Paternity testing Criminal cases Locating disease genes. Criminal Case.

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RFLP

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  1. RFLP • “Restriction Fragment Length Polymorphism” • Basic idea: • If two DNA sequences have different numbers or locations of restriction sites, they will differ in their restriction enzyme digest pattern • Uses: • Paternity testing • Criminal cases • Locating disease genes

  2. Criminal Case http://www.scientific.org/case-in-point/articles/thompson/

  3. Paternity/Maternity testing Child 2 Child 1 Dad Mom

  4. Recombinant DNA • Engineered pieces of DNA inserted into the genomes of a target organisms • Can be used to mass produce useful proteins • “Humulin” – human insulin produced by bacteria (Eli Lilly company) • Human growth hormone • Also used to modify organisms • All originally depends on restriction enzymes!

  5. Gene cloning with restriction enzymes

  6. GloFish

  7. Why do bacteria not cut their own genomes? • They protect their DNA with methylation Restriction enzymes can’t cut here

  8. Optical Mapping Image of single molecule of DNA after being cut by restriction enzymes EagI & MluI Laboratory of David Schwartz, UW-Madison

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