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M3: Do a conclusion for investigation of contaminated river water. M3: Conclusion. Questions to address: Does the river water contain the safe purple dye or the dangerous purple haze? What evidence do you have to back up your answer (Refer to chromatogram- refer to the spots)
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M3: Do a conclusion for investigation of contaminated river water
M3: Conclusion • Questions to address: • Does the river water contain the safe purple dye or the dangerous purple haze? • What evidence do you have to back up your answer (Refer to chromatogram- refer to the spots) • Does the river water contain purple haze that is below or above the legal limit? • What evidence do you have to back up your answer (Refer to colorimetry graph- refer to the absorbance value and concentration of the river water and the concentration “750ppm”)
Chromatography Why did we use chromatography? Why did we use “river water”? Why did we use “safe purple dye”? Why did we use “purple haze”? Why did we use filter paper? Why did we use water?
Chromatography Why did we use the glass spotters? Why did we use splint and paper clip? Why did we leave the filter paper in the beaker of water for a while? Why did we measure the distances that the components moved and solvent front? Why did we calculate Rf values?
Colorimetry Why did we use colorimetry? Why did we measure absorbance values of different wavelengths on the river water? Why did we use “known” different concentrations of purple haze? Why did we use curvettes? Why did we use dropping pipettes?
Colorimetry Why were the different concentrations of purple haze in 100ml volumetric flask? Why did we use a colorimeter? Why did we plot a graph of the absorbance values against the concentration of the “known” solutions? Why did we do a line of best fit on the graph?
Colorimetry Why did we compare the concentration of river water to the value “750ppm”?