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Enzymes

Lab 5. Enzymes. Chemical reaction. The cells carries out many chemical reactions. A + B C +D reactants products. Enzymatic action. Enzymes are organic catalysts that speed metabolic reactions.

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Enzymes

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  1. Lab 5 Enzymes

  2. Chemical reaction • The cells carries out many chemical reactions. A + B C +D reactants products

  3. Enzymatic action • Enzymes are organic catalysts that speed metabolic reactions. • Most of the enzymes are proteins. • The reactants in an enzymatic chemical reaction are called substrates. E + S ES E + P

  4. Enzymes have specific shape accommodating the shapes of their substrate. • Enzymes can be used over and over again. Active site

  5. Enzyme's optimal conditions • The rate of an enzymatic reaction depends on conditions in the immediate environment • These conditions affect the shape of the enzyme and modify the active site and precise fit of an enzyme and its substrate • Factors influencing enzyme functions include temperature, PH, concentration of substrate and enzyme.

  6. Enzyme denature • Any structural change in an enzyme may denature or destroy its effectiveness by altering the active site and slowing down the reaction. • Denatured enzymes may result from extreme temperature, extreme PH, or any environmental condition that fundamentally alters a protein's structure.

  7. Goals of today's experiment • To examine enzyme specificity • To determine the optimal temperature for sucrosase • To determine the optimal PH for sucrosae • To compare enzyme performances under different concentrations

  8. Following handout

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