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ANSWERS FOR QUIZ. Set-1. Answers to Bacterial gel. 1.760 2. 245 3. 16 spots 4.142/760 x 100 i.e.18.68% 5. 71-26 = 45. 6. 20 spots 7. 20/760 x 100 i.e.2.63% 8. 47-12 = 35. 9.(i) Spot ID: 2082 pI : ~4.53 Mol. weight : 24.9 kDal.
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Set-1. Answers to Bacterial gel 1.760 2. 245 3. 16 spots 4.142/760 x 100 i.e.18.68% 5. 71-26 = 45. 6. 20 spots 7. 20/760 x 100 i.e.2.63% 8. 47-12 = 35. 9.(i) Spot ID: 2082 pI : ~4.53 Mol. weight : 24.9 kDal (ii) Spot ID: 1999 pI : ~4.90 Mol. weight : 75.2 kDal
1. Project Summary: Groups Control(1 image) 780spots transferred from fused image 96 spots cancelled Drug Treated(1 image) 780 spots transferred from fused image 96 spots cancelled Vector Matching :245
3.Figure showing Molecular weight to pI pI index Molecular weight (kDal) pI : ~4.53 Mol. weight : 24.9 kDal pI : ~4.90 Mol. weight : 75.2 kDal
4.1.Differential expression : Up regulation Up regulation x Folds or above No. of spots showing up regulation
4.2.Differential expression : Down regulation Down regulation x Folds or above No. of spots showing up regulation
Set -1. Answer of Serum sample 1. 349 spots 2. 109 3. 16 spots 4. 69/349 x 100 i.e.19.77% 5. 33-16 = 17 6. 28 spots 7. 48/349 x 100 i.e.13.75 % 8. 83-28 = 55 9.(i) Spot ID: 2082 pI : 5.28 Mol. weight : 44.8 kDal (ii) Spot ID: 1999 pI : ~5.26 Mol. weight : 65.2 kDal
Project Summary: Groups Control(1 image) Control - Healthy person 349spots transferred from fused image 97 spots cancelled Diseased (1 image) P.falciparum diseased serum sample 349 spots transferred from fused image 97 spots cancelled Vector Matching : 109
3.Figure showing Molecular weight to pI pI index Molecular weight (kDal) pI : 5.28 Mol. weight : 44.8 kDal pI : 5.26 Mol. weight : 65.2 kDal
Differential expression : Up regulation Up regulation x Folds or above No. of spots showing up regulation
4.2.Differential expression : Down regulation Down regulation x Folds or above No. of spots showing up regulation