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Laboratory Eleven

Laboratory Eleven. The Circulatory System: Physiology. The Electrocardiogram: Recording of Heart Electrical Activity (p202, 11A Procedure, #2). P wave Atrial depolarization Occurs before atrial contraction Generated by the firing of Sinoarteial (SA) node QRS complex

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Laboratory Eleven

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  1. Laboratory Eleven The Circulatory System: Physiology

  2. The Electrocardiogram: Recording of Heart Electrical Activity(p202, 11A Procedure, #2) • P wave • Atrial depolarization • Occurs before atrial contraction • Generated by the firing of Sinoarteial (SA) node • QRS complex • Ventricular depolarization • Occurs before ventricular contraction (systole) • T wave • Ventricular repolarization • Occurs before ventricular relaxation (diastole)

  3. Pulse & Blood Pressure(p203, 11B Procedure) • Determine pulse rate manually in 5 different locations • Measure systemic blood pressure using the peripheral pulses in the brachial artery • Systolic pressure (first soft sound): generated by contraction of left ventricles • Diastolic pressure (sound terminates): corresponds to relaxation of the left ventricle

  4. Blood Typing

  5. Anti-B B Rh Anti-Rh A Anti-A Blood Typing(p205, 11C Procedure) • 2 drops of the synthetic blood of only one subject • 2 drops of each anti-serum • Mix with toothpicks (avoid cross contamination) • Place the slide on the Rh-typing box for 30-45sec while gently rocking the box • Observe the wells for agglutination (RBC clumping) • Record your results in your lab report • e.g.: • B- (agglutination in anti-B well) • O+ (agglutination in anti-Rh well) • AB+ (agglutination in Anti-A, -B,& -Rh wells)

  6. A Typical Antibody

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