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Relative SOD1-G93A expression In slow and fast muscle. SOD1-G93A males. 1,40. P=0.571. 1,20. 1,00. 0,80. Relative expression. 0,60. 0,40. 0,20. 0,00. SOLEUS. EDL. Supplementary Figure 1 hSOD1-G93A is equally expressed in cultured primary myoblasts
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Relative SOD1-G93A expression In slow and fast muscle SOD1-G93A males 1,40 P=0.571 1,20 1,00 0,80 Relative expression 0,60 0,40 0,20 0,00 SOLEUS EDL Supplementary Figure 1 hSOD1-G93A is equally expressed in cultured primary myoblasts derived from soleus (slow) and EDL (fast) muscles. The data shows mean (+/- standard error of mean) expression of human SOD1 from four replicate satellite cell cultures of hSOD1-G93A transgenics. No hSOD1 expression was observed in satellite cell cultures derived from wild-type animals.
Supplementary Figure 2Satellite cell cultures from EDL and soleus muscles of SOD1G93A transgenic mice. Myogenin expression was analysed by immunocytochemistry after induction of differentiation 4.5 days post plating.Nuclear Hoechst staining from A EDL and C SOL. Myogenin staining from B EDL and D SOL.