1 / 10

Seeding

Seeding. Terese Bergfors Dept of Cell and Molecular Biology Uppsala University, Uppsala Sweden terese@alpha2.bmc.uu.se Examples of two new structures from our laboratory where seeding was critical to obtaining the crystals. Seeding. Why seed? How? What to use as a seed?.

dionne
Download Presentation

Seeding

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Seeding Terese Bergfors Dept of Cell and Molecular Biology Uppsala University, Uppsala Sweden terese@alpha2.bmc.uu.se Examples of two new structures from our laboratory where seeding was critical to obtaining the crystals

  2. Seeding • Why seed? • How? • What to use as a seed?

  3. Some methods for optimization microgravity seeding

  4. Seeding and the phase diagram Luft and De Titta, Acta Cryst. (1999) D55, 988-993

  5. Serial seeding 20 mg/ml 10 mg/ml 10 mg/ml

  6. What to seed from? oil Take your best crystal or any solid phase

  7. Streak seeding whisker Touch crystal Draw streak line Enrico Stura in “Crystallization of Nucleic Acids and Proteins” Ed. Ducruix and Giege, 1992 Oxford University Press p. 108

  8. Does seeding solve everything? before after

  9. After Seeding, Ni Does seeding solve everything? After seeding, Ni, and ligand

  10. Summary of Tips • Lower concentration • Use any solid phase • Serial seed • Use sitting drops

More Related