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a. X. N. X. N. H. H. pduP. T7. pET28a. pGEM-T Easy- pduP. ( N + X ). N. X. pduP. H. T7. pET - pduP. b. X b. B. X b. X. B. X. pduP. P. pGEM-T Easy-P lacZ. pGEM-T Easy- pduP. (X b +B). X b. X. B. X. X. pduP. P. dhaT. P. orf13. orf14. P. pVOT.
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a X N X N H H pduP T7 pET28a pGEM-T Easy-pduP (N+X) N X pduP H T7 pET-pduP b Xb B Xb X B X pduP P pGEM-T Easy-PlacZ pGEM-T Easy-pduP (Xb+B) Xb X B X X pduP P dhaT P orf13 orf14 P pVOT pGEM-T Easy-P-pduP (X) X X P dhaT P pduP P orf13 orf14 pVOTLp Supplementary Fig 1