Chapter 28 Reading Quiz

1. Chapter 28 Reading Quiz • Which kingdom is the most diverse of all eukaryotes? • In which kingdom do algae belong? • Through what process is it proposed that mitochondria & chloroplasts came to reside within larger cells? • Plasmodium causes what (at this time) incurable disease? • The suffix “-phyta” refers to what main type of protist?

2. 1. List the characteristics of protists. • They are the earliest eukaryotes  showed up a billion years before the others • Age  2.1 billion years • There are 60,000 living species • Exist as unicellular, colonial, and multicellular • Aerobic, cilia & flagella, asexual or sexual • Bottom line: a VERY diverse group 

4. 2. Explain why some biologists prefer to use the term undulipodia for eukaryotic flagella and cilia. • Because they appear to “undulate” or wave while acting like feet or “podia” 

5. 3. Briefly summarize and compare the two major models of eukaryotic origins, the autogenous hypothesis and the endosymbiotic hypothesis. • Specialization of the plasma membrane invagination  gave rise to the nuclear envelope, the ER, the Golgi apparatus, etc… • Endosymbiotic associations may have resulted in organelles  mitochondria, chloroplasts, etc… 

7. Secondary endosymbiosis

9. 4. Provide three major lines of evidence for the endosymbiotic hypothesis. • Similarities between organelles and prokaryotes • Molecular systematics lends support  rRNA of chloroplasts is more similar in base sequence to RNA from certain eubacteria than rRNA in eukaryotic cytoplasm • 9 + 2 flagella and cilia are analogous to prokaryotes 

11. 5. Explain why modern biologists recommend expanding the original boundaries of the Kingdom Protista. • Observation that the Kingdom Protista is polyphyletic • Current research groups protists into FIVE candidate Kingdoms 

12. 6. Explain what is meant by the statement that the Kingdom Protista is a polyphyletic group. • Polyphyletic = ancestry from several possible sources or directions • Example: Protists are  animal-like plant-like fungus-like 

14. 7. List five candidate kingdoms of protists and describe a major feature of each. • Archaezoa  lack mitochondria • Euglenozoa  are both autotrophic and heterotrophic flagellates • Alveolata  have subsurface cavities (alveoli) • Stramenopila  diatoms, golden brown algae, and water molds • Rhodophyta  red algae; lack flagella 

15. 8. Describe amoeboid movement. • Amoebas move in a characteristic motion • use “pseudopodia”  form as cellular extensions and function in feeding and movement • The cytoskeleton of microtubules and microfilaments functions in this amoeboid movement 

17. 9. Outline the life cycle of Plasmodium. • It is the protist that causes malaria • The Anopheles mosquitos serve as the intermediate host and humans are the final host • The Plasmodium spends most of its life in blood or liver cells 

19. 10. Indicate the organism that causes African sleeping sickness and explain how it spread and why it is difficult to control. • It is a species of Trypanosoma that causes the disease and are spread by the bite of the tsetse fly • It belongs in the candidate Kingdom Euglenozoa, in the group Kinetoplastids 

21. 11. Describe the function of contractile vacuoles in freshwater climates. • Contractile vacuoles allow freshwater protists to: 1. Maintain water balance and homeostasis 2. Expel accumulated water from osmosis 3. Sometimes helps propel protists 

22. 12. Distinguish between macronuclei and micronuclei. • Macronuclei large, over 50 copies of genome - controls everyday functions of the cell by synthesizing RNA - necessary for asexual reproduction during binary fission • Micronuclei  small, about 1 – 80 of these - no function in growth, maintenance, or asexual reproduction - functions only in conjugation (sexual genetic variation) 

23. 13. Using diagrams, describe conjugation in Paramecium caudatum. • Page 559 in your book • Two paramecium have diploid micronuclei which go through meiosis to create haploid micronuclei • Through syngamy, the two paramecium exchange a couple haploid micronuclei, and these fuse to become a new, varied, diploid micronucleus • The new diploid micronuclei may also then follow the cycle and exchange with another paramecium throughout it’s life cycle 

26. 14. Explain how accessory pigments can be used to classify algae and determine phylogenetic relationships among divisions. • Diatoms  brown plastids cause brown/yellow color • Golden algae  yellow and brown carotenoids and xanthophyll • Brown algae  chlorophyll a, c and the carotenoid fucoxanthin • Red algae  chlorophyll a, carotenoids, phycobilins, and chlorophyll d - color is due to accessory pigment phycoerythrin 

28. 15. In a chart, distinguish among the following algal groups based on their pigments, cell wall components, storage products, reproduction, number and position of flagella, and habitat:a. Dinoflagellata b. Bacillariophytac. Chrysophyta d. Phaeophyta

29. 16. Describe three possible evolutionary trends that led to multicellularity in the Chlorophyta. • Formation of colonies of individual cells ex: Volvox • Repeated division of nuclei with no cytoplasmic division (mitosis without cytokinesis ex: Caulerpa • Formation of true multicellular forms ex: Ulva

30. volvox

31. caulerpa

32. Life Cycle overview • Sporophyte  generally the adult form of an organism; exists as a diploid (2n); makes spores (ex: humans) • Gametophyte  generally an intermediate step before fertilization; exists as a haploid (n); makes gametes (ex: sperm & eggs) 

33. 17. Outline the life cycles of Chlamydomonas, Ulva, and Laminaria and indicate whether the stages are haploid or diploid. • Chlamydomonas at maturity it is a single haploid cell - asexual at first, then sexual only if stressed, creating a diploid zygote 2. Ulva diploid sporophyte and haploid gametophyte 3. Laminaria  sporophyte (2n) with sporangia makes zoospores (n) which make gametes (n) and then fertilization to get diploid sporophyte again 

35. 18. Distinguish between isogamy and oogamy; sporophyte and gametophyte; and isomorphic and heteromorphic generations. • Isogamy  when gametes are morphologically indistinguishable • Oogamy  flagellated sperm fertilize the nonmotile egg • Sporophyte  2n (diploid) generation • Gametophyte  n (haploid) generation • Isomorphic  gametophytes and sporophytes look alike • Heteromorphic  gametophytes and sporophytes are structurally different 

36. Plasmodial Slime Mold Multinucleated mass Diploid nuclei When stressed will form sexual reproductive structures called sporangia  Cellular Slime Mold Solitary haploid cells Cells will aggregate when food supply is low Fruiting bodies (sporangia) function in asexual reproduction  19. Compare the life cycles of plasmodial and cellular slime molds and describe the major differences between them.

39. 20. Provide evidence that the oomycetes are not closely related to true fungi. • Have coenocytic (multinucleated) hyphae (branching filaments) that are analogous to fungal hyphae • Cell walls are made of cellulose rather than the chitin in true fungi • Have biflagellated cells  fungi lack flagellated cells 

40. 21. Give examples of oomycetes and describe their economic importance. • Water molds  grow on injured tissue but also will grow on the skin and gills of fish • White rusts • Downy mildews - both of these are parasitic on terrestrial plants – act as pathogens 

42. 22. Explain the most widely accepted hypothesis for the evolution of multicellularity. • Multicellularity arose from unicellular ancestors as colonies or loose aggregates of interconnected cells • This involved cellular specialization and division of labor among the cells The End 