1 / 7

Exploring Dopa Oxidase: Perfect Enzyme Characteristics & Practical Application Guide

Discover the key characteristics of a perfect enzyme for practical use, such as robustness, versatility, and reliability. Learn how to assay Dopa oxidase with a simple and quick method for kinetic studies.

dlucas
Download Presentation

Exploring Dopa Oxidase: Perfect Enzyme Characteristics & Practical Application Guide

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Dopa oxidase – the perfect enzyme? Paul Beaumont / Kate Andrews Kath Crawford / Lorraine Bruce

  2. What characteristics do you look for in an enzyme practical?

  3. Robust • Cheap • Versatile • Reliable • ‘Living thing-based’ • Easy to assay • Quick • Amenable to kinetic studies • Open to investigations e.g. temperature, pH

  4. Tyrosinase / dopa oxidase / catechol oxidase Adapted from http://mystrica.com/

  5. Reaction

  6. As per instruction sheet. Materials

  7. Banana ~ 2.5 g + 5.0 mL cold H2O Centrifuge 2 min – enzyme extract Zero colorimeter at 470 nm In cuvette: 200 mL extract + 800 mL H2O Place in colorimeter Add 2.0 mL L-dopa solution and start stop-clock. Measure colour every 20 s (or so...) The Assay

More Related