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Dopa oxidase – the perfect enzyme? Paul Beaumont / Kate Andrews Kath Crawford / Lorraine Bruce

Dopa oxidase – the perfect enzyme? Paul Beaumont / Kate Andrews Kath Crawford / Lorraine Bruce. What characteristics do you look for in an enzyme practical?. Robust Cheap Versatile Reliable ‘Living thing-based’ Easy to assay Quick Amenable to kinetic studies

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Dopa oxidase – the perfect enzyme? Paul Beaumont / Kate Andrews Kath Crawford / Lorraine Bruce

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  1. Dopa oxidase – the perfect enzyme? Paul Beaumont / Kate Andrews Kath Crawford / Lorraine Bruce

  2. What characteristics do you look for in an enzyme practical?

  3. Robust • Cheap • Versatile • Reliable • ‘Living thing-based’ • Easy to assay • Quick • Amenable to kinetic studies • Open to investigations e.g. temperature, pH

  4. Tyrosinase / dopa oxidase / catechol oxidase Adapted from http://mystrica.com/

  5. Reaction

  6. As per instruction sheet. Materials

  7. Banana ~ 2.5 g + 5.0 mL cold H2O Centrifuge 2 min – enzyme extract Zero colorimeter at 470 nm In cuvette: 200 mL extract + 800 mL H2O Place in colorimeter Add 2.0 mL L-dopa solution and start stop-clock. Measure colour every 20 s (or so...) The Assay

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