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Microbial Functional Genomics

Microbial Functional Genomics. S46964117 呂凱筠. Functional Genomics. Microarray Mutation analysis Proteomics. The Microarray Assay and Differential Display Proteomics. For global analysis Changes in the environment Regulatory gene knock out Strains Response to host cells. Microarray.

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Microbial Functional Genomics

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  1. Microbial Functional Genomics S46964117呂凱筠

  2. Functional Genomics • Microarray • Mutation analysis • Proteomics

  3. The Microarray Assay and Differential Display Proteomics For global analysis • Changes in the environment • Regulatory gene knock out • Strains • Response to host cells

  4. Microarray

  5. Suppression Subtractive Hybridization (SSH) • SSH provides a 10-100 fold enrichment of differentially expressed mRNAs.

  6. Nature Reviews Genetics 7:929-939 (2006)

  7. Signature Tagged Mutagenesis (STM) Nature Reviews Genetics 7:929-939 (2006)

  8. In vivo expression technology (IVET))

  9. Differential fluorescence induction (DFI)

  10. Transposon-Site Hybridization

  11. Transposon-Site Hybridization Rich medium Minimal medium

  12. Fluorescence Two-dimensional Difference Gel Electrophoresis: 2D DIGE

  13. A protocol for high-throughput protein purification (His-tag) 4256 His-tagged protein constructs in E. coli OD600≒0.7 3.5 hours

  14. Immobilization SDS-PAGE Proteome chip Coomassie stain His6 mAb + Cy5-labeled secondary antibody

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