1 / 25

Shan-Jing Geng, et al. 2010

Contribution of the spinal cord BDNF to the development of neuropathic pain by activation of the NR 2B -containing NMDA receptors in rats with spinal nerve ligation. Shan-Jing Geng, et al. 2010. “brain food”. -~ BDNF ~-. BDNF added. Control. Receptor for BDNF Involved in synaptogenesis

domingoe
Download Presentation

Shan-Jing Geng, et al. 2010

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Contribution of the spinal cord BDNF to the development of neuropathic pain by activation of the NR2B-containing NMDA receptors in rats with spinal nerve ligation Shan-Jing Geng, et al. 2010

  2. “brain food” -~BDNF~- BDNF added Control

  3. Receptor for BDNF • Involved in synaptogenesis • More receptors, more response - TrkB -

  4. NMDA • Calcium channel involved in plasticity Ca2+ Glutamate

  5. NMDA • Channels made out of 4 subunits • NR2 has different isoforms: NR2 A, B, C, D Today we care about NR2B Ca2+ NR1 NR2 Glutamate

  6. NMDA • NR2B • In the context of neuropathic pain it Potentiates the signal CAUSING pain • Can’t have neuropathic pain w/o NR2B • (It’s wicked important)

  7. The mechanism BDNF NMDA channel TrkB receptor NR2B NR2B

  8. “Chain of Pain” Brain • Pain receptor • Mechanical • polymodal Spinothalamic tract Dorsal Horn: Lamina I & II C & A δ Fibers

  9. “Chain of Pain” Light Brain Spinothalamic Tract Dorsal Horn C & Aδ Fibers Pain Receptor

  10. Dorsal Horn was examined • Immunohistochemistryfor BDNF & NR2B • Lamina I & II • ElisaKitfor BDNF • Dorsal horn • Western for TrkB • Dorsal horn

  11. Spinal Nerve Ligation (SNL) Poor Rats  • Induces neuropathic pain Intrathecal Catheter Implant Makes for a precise injection

  12. ?

  13. Von Frey Filiments

  14. R2=0.94 Early Late R2=0.11

  15. BDNF NMDA channel TrkB receptor NR2B NR2B

  16. BDNFimmunohistochemical

  17. Follows the pattern of BDNF TrkB

  18. NR2Bimmunohistochemical

  19. BDNF NR2B

  20. NR2B Blocker (antagonist) • Co-administered with BDNF • Does not affect BDNF binding or signaling Ro 25-6981 experiment

  21. Ro BDNF TrkB receptor NR2B NR2B

  22. NR2B is essential to BDNF mediated neuropathic pain • BDNF initiates Neuropathic pain • NR2B keeps it going • Why does pain stay when NR2B goes back down? • AMPA Almost there..

More Related