1 / 22

Enteric Pathogens

Enteric Pathogens. Beth Schweitzer MT(ASCP), SM Nebraska Public Health Laboratory. Pathogens Routinely Tested. Salmonella spp. Shigella spp. Campylobacte r spp. Aeromonas Plesiomonas Vibro. Pathogens Tested for upon request. Yersinia entercolitica E. coli 0157:H7 Vibrio

don
Download Presentation

Enteric Pathogens

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Enteric Pathogens Beth Schweitzer MT(ASCP), SM Nebraska Public Health Laboratory

  2. Pathogens Routinely Tested • Salmonella spp. • Shigella spp. • Campylobacter spp. • Aeromonas • Plesiomonas • Vibro

  3. Pathogens Tested for upon request • Yersinia entercolitica • E. coli 0157:H7 • Vibrio • Must be specifically requested

  4. Testing for Salmonella/Shigella • Look for appearance of colonies on specialized media

  5. Normal Enteric Flora

  6. Salmonella/ Shigella

  7. E. coli/ Shigella

  8. Salmonella

  9. Campylobacter testing • Look for colonies on media after incubation at specific temperatures and atmospheric conditions

  10. Campylobacter screening

  11. Campylobacter spp.

  12. Gram- Negative cell structure

  13. Salmonella Serogrouping • Based on Cell Wall Antigens • (O) Somatic • Each antigen identified by a number designation • Multiple antigens can be present on each cell’s surface • Serogroup is determined by pattern of O antigens present • 95% of isolates belong to serogroups A-E (O2-O10) • Remaining 5% O antigens O11-O67 • Commercial kits available • Latex agglutination

  14. Salmonella serotyping • Based on Flagellar antigens (H) • Each antigen identified by a letter or number designation • Multiple antigens can be present on each isolate • Most isolates can posses 2 sets (phase1/phase 2) • Serotype is determined by O antigens present and phase 1/phase 2 H antigens • Over 2400 different serotypes • Over 1400 common to humans • No commercial kit available

  15. Serotyping • End up with an antigenic formula • O antigen: Phase 1 H antigens: Phase 2 H antigens • Most common formula’s have a name • Salmonella serotype Typhimurium • (4,5:i:2) • Serogroup B • Salmonella serotype Enteritidis • (9:gm:-) • Serogroup D

  16. E. coli O157:H7 • First look for E. coli in stool • Sorbitol negative • Test organism for presence of O and H antigens • Commercial kit available

  17. Further testing • All E coli O157:H7 isolates have Pulse field gel electrophoresis (PFGE) performed on them • Any other organisms can have PFGE performed, at the request of the state

  18. Any questions??Contact informationbschweitzer@unmc.edu559-6098

More Related