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靜磁場對於 PC12 類神經細胞之神經生長影響

靜磁場對於 PC12 類神經細胞之神經生長影響.

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靜磁場對於 PC12 類神經細胞之神經生長影響

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  1. 靜磁場對於PC12類神經細胞之神經生長影響 諸多研究指出，鈦金屬與其合金具有極高的生物相容性，非常適合做為人體的植入物，然而鈦金屬與其合金之所以具極佳的生物相容性(Biocompatibility)主要是與其表面的氧化層有關，研究指出植體表面氧化層厚度與錶面結構對於細胞初始的攀附行為、增殖及分化有密切的關係。而本研究將利用氧氣電漿鈦金屬表面做氧化處理，並使其表面呈現奈米化結構，藉此表面處理來提高鈦金屬之生物相容性。而結果顯示，經電漿處理後之表面氧化層，其相由α相 轉變為 α + TiO 再轉變為α + TiO + ??-TiO2，這樣的結果是於先前研究中沒發現過的，而??-TiO2可有效提高植體的骨整合。此外，氧化層中形成的奈米TiO + ??-TiO2相之奈米結構可提高蛋白質的貼附，藉此提高細胞的貼附及血液反應，藉此提高生物相容性，並有效提高骨整合。

  2. The Influence of Static Magnetic Field on Neuronal Outgrowth of PC12 cells • Previously investigations indicated that pulse electromagnetic field (PEMF) can be a neuroregenerative stimulator for stroke and traumatic injuring patients. It can be a local healing stimulator for nerve regeneration and has potential to become a non-invasive physical therapeutic tool for these patients. Recently, it was found that, similar to PEMF, static magnetic field (SMF) can promote osteoprogenitors differentiate into mature phenotype. Accordingly, in this study proposal, we hypothesize that SMF can also be a non-invasive stimulator to promote nerve regeneration. To test this hypothesis, PC12 cells were carried out for in vitro experiments. The cells were pre-induced to express neural characteristics with 25 ng/ml and 50 ng/ml nerve growth factor (NGF). Then the induced cells were continuously exposed to 4000G SMF for 15 days. Cell morphologies were observed by optical microscope at 5, 10 and 15 days after SMF treatment. The images of the cells were recorded by a digital camera at the observation times. In addition, we compared the morphology changes of the differentiated PC12 cells treated with and without SMF exposure by counting the length and branching degree of dendrites.Our preliminary results showed that SMF-treatment has neurite-elongation effect in the NGF-induced PC12 cells. These results suggest that the SMF may affect neurogenerative process at the second stage, i.e, neurite elongation. When the cells were exposed to SMF, the population of the cells extending neurites of length more than once, twice and thrice the cell diameter, the datum were 94.84±4.51%、74.05±13.09% and 57.04±6.45%, respectively. However, the analogous value of the unexposed cells of the different multiple were taken into account, these results were significantly reduced to 74.76±5.16%、48.52±8.48% and 31.59±11.75% So, base on these findings, additional experiments on the mechanism of such effect should be valuable for more insight into the application of SMF-induced nerve regeneration or nerve differentiation.

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