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Functionalisation of whole cell bacterial reporters with magnetic nanoparticles supplement figures

Functionalisation of whole cell bacterial reporters with magnetic nanoparticles supplement figures. Dayi Zhang 1,  , Rawil F. Fakhrullin 2,3,  , Mustafa Özmen 3,4 , Hui Wang 5 , Jian Wang 6 , Vesselin N. Paunov 3 , Guanghe Li 7 and Wei E. Huang 1, *.

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Functionalisation of whole cell bacterial reporters with magnetic nanoparticles supplement figures

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  1. Functionalisation of whole cell bacterial reporters with magnetic nanoparticlessupplement figures Dayi Zhang 1, , Rawil F. Fakhrullin 2,3, , Mustafa Özmen3,4, Hui Wang 5, Jian Wang 6, Vesselin N. Paunov3 , Guanghe Li7 and Wei E. Huang 1, *

  2. Figure S1. SEM image of Acinetobacter ADPWH_lux bioreporters functionalised with PAAH-stabilised MNPs.

  3. A Figure S2. The efficiency of MNPs functionalising Acinetobacter strain ADPWH_lux was 99.96 ±0.01% . Cell populations of magnetic and non-magnetic cells after MNPs functionalisation were 2.22×108 and 8.00×104 separately. The process of magnetic functionalisation was biocompatible since magnetic cells were still viable and able to form colonies on LB agar plates.

  4. B Figure S3. Dynamics of non-magnetic and magnetic Acinetobacter strain ADPWH_lux during 120 min incubation. Initially, all cells were magnetic. Non-magnetic free cells appeared after 30 min incubation due to bacterial division and they gradually increased to 12% at 120 min.

  5. A B Native cells MNPs functionalised cells Figure S4. Calibration curves of OD600 and cell population of (A) native Acinetobacter cells and (B) MNPs functionalised Acinetobacter cells when cells were grown in MMS medium.

  6. A B Figure S5. The bioluminescence intensity (data averaged from 40-90 min in Fig. 4) of ADPWH_lux is proportional to the concentration of salicylate in sediments (A) and soils (B) in the range of 0-14 mg/kg samples.

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