HEAT stresS RESPONSE and Heat-shock Protein IN PLANTS

1. HEAT stresS RESPONSE and Heat-shock Protein IN PLANTS By Kelompok 9 : Agatha Maturbongs : 12/337941/PBI/1048 Nurhening Yuni Ekowati : 12/338474/PBI/1069

2. Plants : Plants as sessile organisms are exposed persistently changing stress factors. Primary stress such as drought, salinity, cold and hot temperatures and chemicals are interconnected in their effect on plants.These factors cause damaged to the plant cell and lead to secondary stresses such as osmotic and oxidative stresses. Plants adapt morphologically and physiologically and biochemically

3. How about heat stress? Active plants growth in mesophile organisms is 10-40oC. Any temperature which is under and below that range creates heat stress on metabolic activities of plants.

5. High temperature morpho-anatomical, physiological and biochemical changes in plants Plants growth and development Reduction in economic yield

6. Plants heat stress

7. Heat stress response is a reaction when tissues and cells of an organism were exposed to sudden heat stress. HS response is initiated by the activation of heat shock factors (HSF) which cause expression of heat shock gene to synthesis heat shock proteins Heat shock proteins (HSPs) are spesific protein that are made when cells are briefly exposed to temperature above their normal growth temperature. Heat stress respon and HSPs are highly conserved in every organism. HSPs can also be induced other stressor such as oxidants, toxins, heavy metals, free radicals, and viruses, so they are sometimes called the “stress protein”

8. Most heat shock proteins are chaperon, they protecting plants againt stress by reestablishing normal conformation and thus cellular homeostasis. HSF is a regulator in HSPs synthesis. These proteins are grouped in plants into five classes according to their approximate molecular weight : • Hsp100 • Hsp90 • Hsp70 • Hsp60 • sHsps

9. Arabidopsis, the genetic model for studying and manipulating the heat stress response. Transgenic overexpression of Arabidopsis thaliana heat shock transcription factor (HSF) results in a synthesis of HSPs at normal temperature and significantly increased basal thermotolerance.

10. HSF expression in tomato plant

11. HSF expression in fruit ripening

12. Plants and animals share similar mechanisms in the heat shock (HS) response, such as synthesis of the conserved HS proteins (Hsps). • In general they require unique features to cope with heat stress. • Hsa32 is a conserved HS-inducible gene in land plants • The gene responds to HS at the transcriptional level in moss (Physcomitrella patens), Arabidopsis (Arabidopsis thaliana), and rice (Oryza sativa).

13. Hsa32 Is a Conserved HS-Inducible Gene in Land Plants • Hsa32 Protein Is Synthesized in Response to HS Charng et al, 2006

14. Wang et al, 2004

15. Wang et al, 2004

16. PUSTAKA • Al-wahibi, M. H. 2010. Plant heat-shock protein : a mini review. Journal of King Sau University xxx: xxx-xxx • Baniwal, S. K, K. Bharti1, K. Y. Chan, M. Fauth, A. Ganguli, S.Kotak, S. K. Mishra, L. Nover, M. Port, K. Scharf, J. Tripp, C. Weber, D. Zielinski and P. V. Koskull-döring. 2004. Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors. J. Biosci. 29(4) : 471-487 • Charng, Yee-yung, Hsiang-chin Liu, Nai-yu Liu, Fu-chiun Hsu, and Swee-suakKo. 2006. Arabidopsis Hsa32, a Novel Heat Shock Protein, Is Essential for Acquired Thermotolerance during Long Recovery after Acclimation. Plant Physiology, Vol. 140, pp. 1297–1305 • Wahid, A., S. Gelani, M. Ashraf, M.R. Foolad. 2007. heat tolerance in plants : an Overview. Environmental and experimental Botany 61 : 199-223 • Wang, Wangxia, BasiaVinocur, OdedShoseyov and Arie Altman. 2004. Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. TRENDS in Plant Science Vol.9 No.5.

17. SEKIAN DAN TERIMA KASIH

18. Pertanyaan-Pertanyaan • Bagaimanahubunganantararusaknyaperikarppadabuahtomat? bagaimanakaitannyadenganglisinbetain? jawab : kerusakan perikarp pada buah tomat disebabkan oleh adanya cekaman panas yang merusak membran sel. Panas menyebabkan perubahan integritas dan fluiditas membran, serta menyebabkan enzim-enzim yang terdapat di dalam sel terdenaturasi sehingga sel menjadi mati. Selain itu dengan adanya cekaman panas menyebabkan sintesis likopen terganggu dan bahkan tidak terjadi, kemungkinan karena enzim yang terlibat dalam sintesis likopen mengalami denaturasi. sedangkan glisin betain merupakan salah satu protein osmoprotektan , peningkatan konsentrasi glisin betain dalam cekaman panas adalah untuk menjaga homeostasis sel, yaitu dengan menjaga tekanan osmosis di dalam sel. • BagaimanakaitanHsf yang dioverekspresikanpadatanaman? Jawab : overekspresi Hsf pada tanaman menyebabkan tanaman lebih toleran terhadap cekaman panas.

19. 3. Apakahpadatumbuhanmemilikisemuajenishsp? Jawab: pada 1 jenistumbuhantidakmemilikisemuajenishsp, masing-masingtumbuhanmemilikihsp yang berbeda-bedatergantungadaptasidanstres yang dialaminya. Misalnya Arabidopsis memiliki 18 gen yang mengkodepembentukan Hsp70 dan Hsp90, Hsp100 dimilikiolehbeberapatanamanantara lain Arabidopsis, kedelaitembakau, padidanjagung. • Hspdihasilkandibagianmanasajapadatumbuhan? Jawab : Hspdihasilkanpadaberbagaiorganelsel, misalnya Hsp70 dihasilkanpadasitosol, kloroplas, danmitokondria, sedangkan Hsp22 dihasilkanpadaretikulumendoplasma.