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Earth Science Institute of Orleans, France UMR 6531 of CNRS - University of Orléans Group « Organic Matter in water, sols, sediments and rocks » Personal involved in RECIPE : Fatima Laggoun Laure Comont (PhD student) Jean-Robert Disnar Patrick Albéric (DOC, water chemistry)
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Earth Science Institute of Orleans, France UMR 6531 of CNRS - University of Orléans Group « Organic Matter in water, sols, sediments and rocks » Personal involved in RECIPE : Fatima Laggoun Laure Comont (PhD student) Jean-Robert Disnar Patrick Albéric (DOC, water chemistry) Christian Défarge (µmorphology - cryo-SEM) Nathalie Lottier (GC-MS / Capillary eletrophoresis) Marielle Hatton (C, N, S & palynofacies preparation) Charquemont, Recipe meeting 23-27th Oct 2003
Appointment of staff and students to RECIPE & relevant problems : Oct. 2001 to Nov. 2002: a PhD student A. Fleury (2 Jura sites) Application for many calls: (i) PhD grants from public Dpts, (ii) Marie Curie post-doc grant (6th Framework Prog.) Oct. 1st 2003: Laure Comont [3 years PhD grant from Research Dpt of Local Adminitr. District (Region Centre)] Eval. MC grant: C. Applicant: Martin Kainz, Univ Victoria, Canada. Project: Biomolecular source identification of OM in peatland ecosystems (lipid biomarkers, i.e. fatty acid methyl esters) Philipp Steinmann: invited staff at ISTO in the framework of RECIPE (1 month (April or May 2004) fund from Univ Orleans). Project: (i) porewater sampling by peepers & isotopic analyses of CO2, CH4 and acetates; (ii) Archaea biomarkers . Charquemont, Recipe meeting 23-27th Oct 2003
Analysis progress: • Sampling on Oct. 2001 • 2 sites in the Jura: • Le Russey (France): 5 cores (70 Samples) • La Chaux d’Abel (Switzerland): 6 cores (91 Samples) • Analyses: CNS analyses, identification & counting of organic fragments, counting of bacteria, sugar analyses of peat samples and vegetation (8 sphagnum sp., 1 polytrichum, 2 Erioph. sp.). • Participation to congresses: 9thCongress of Sedimentology (Oct., 14-16th 2003, Bordeaux) 7th Meeting of Soil Study (Oct., 22-24th 2002, Orleans) Charquemont, Recipe meeting 23-27th Oct 2003
C/N ratios and microscopic counting Chaux d’Abel/fen (minetrophic zone)-Newly restaured CA.1Sphagnum & Polytrichum CA.2Sphagnum & Eriophorum
C/N ratios and microscopic counting Chaux d’Abel/bog (ombrotrophic zone) Newly restaured CA.5Sphagnum & Polytrichum CA.4Sphagnum & Eriophorum Charquemont, Recipe meeting 23-27th Oct 2003
C/N ratios and microscopic counting Chaux d’Abel Intact zone (CA 6) Charquemont, Recipe meeting 23-27th Oct 2003
C/N ratios and microscopic counting (Le Russey) Ry.B – no regeneration ? Ry.D – more advanced stage Regeneration ?
C/N ratios and microscopic counting (Le Russey) Ry.I –Intact peat Regeneration Charquemont, Recipe meeting 23-27th Oct 2003
vegetation % ERIOPHORUM Xylose Arabinose Xyl Ara ERIOPHORUM % POLYTRICHUM Man H Mannose H POLYTRICHUM % S. fallax % Rhamnose Galactose S. rubellum SPHAIGNES Gal Gal Rhm Rhm Biomarkers of organic sources(sugar analyses) Charquemont, Recipe meeting 23-27th Oct 2003
La Chaux d’Abel / bog - exploited zone % Mannose Profondeur (cm) % Arabinose et Xylose 3 3 % Rhamnose et Galactose Profondeur (cm) 2 2 Profondeur (cm) 1 Xylose Arabinose ERIOPHORUM POLYTRICHUM Mannose H 1 Rhamnose Galactose SPHAIGNES sugar analyses Charquemont, Recipe meeting 23-27th Oct 2003
% Xylose % Man, Ara, Fuc et Rib % Glucose Profondeur (cm) Profondeur (cm) 2 1 2 Profondeur (cm) 1 1 Xylose Arabinose ERIOPHORUM POLYTRICHUM Mannose H Rhamnose Galactose SPHAIGNES La Chaux d’Abel / INTACT ZONE sugar analyses Charquemont, Recipe meeting 23-27th Oct 2003
bacteria counting 50 m 3 m 5 m Observations x1000 en épifluorescence Charquemont, Recipe meeting 23-27th Oct 2003
EXPLOITED Zone INTACT Zone Abondance b/g.ech.sec. Biomasse mgC.g Abondance b/g.ech.sec. Biomasse mgC.g Profondeur (cm) Profondeur (cm) Profondeur (cm) Profondeur (cm) bacteria counting (La chaux d’Abel) Taille échantillon de départ Concentration en bactéries/g de tourbe fraîche BIOMASSE Carbone Biovolume CA 5 CA 6 Charquemont, Recipe meeting 23-27th Oct 2003
Bacteria counting (Le Russey) Ry.B – Exploited zone Charquemont, Recipe meeting 23-27th Oct 2003
Bacteria counting (Le Russey) Ry.D – Exploited zone Regeneration Transition (C/N, %OF) Charquemont, Recipe meeting 23-27th Oct 2003
Bacteria counting (Le Russey) Ry.I – Intact zone Regeneration Charquemont, Recipe meeting 23-27th Oct 2003
Replicates ? (Ry A & B: less advanced regeneration stage) Charquemont, Recipe meeting 23-27th Oct 2003