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find best match. find best match. reference data base. experimental data. GenBank Entry A (defined by polygon of sequence derived tRFs). Alu I. add A to results. peak Alu.1. Alu I. find best match. calculate score. peak Alu.2. Yes. peak Alu.3. Bme 1390I. match > 2/3 ?.
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find best match find best match reference data base experimental data GenBank Entry A(defined by polygon of sequence derived tRFs) AluI add A to results peak Alu.1 AluI find best match calculate score peak Alu.2 Yes peak Alu.3 Bme1390I ... match > 2/3 ? calculate score score sum Bsh1236I Bme1390I No calculate score peak Bme.1 discard A peak Bme.2 peak Bme.3 GenBank Entry B(defined by polygon of sequence derived tRFs) ... add B to results AluI find best match calculate score Yes Bsh1236I Bme1390I match > 2/3 ? peak Bsh.1 calculate score find best match score sum peak Bsh.2 Bsh1236I peak Bsh.3 No calculate score find best match ... discard B (+ additional enzymes, up to 13)