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Renal biopsy. Microdisection of tubulointerstitial and glomerular tissue. Transcriptomics: mRNA. Bioinformatics: selection of candidate genes. RT-PCR: mRNA expression confirmation. Inmunohistochemistry: protein expression localization/confirmation. Functional studies in cultured cells.
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Renal biopsy Microdisection of tubulointerstitial and glomerular tissue Transcriptomics: mRNA Bioinformatics: selection of candidate genes RT-PCR: mRNA expression confirmation Inmunohistochemistry: protein expression localization/confirmation Functional studies in cultured cells Functional studies in animal models Clinical studies Figure 1
Osteoprotegerin TRAIL 14-3,3 eta CD74 GG2-1 Osteopontin Caspase 1 EIF4G2 TIA1 Clusterin STAT1 Tubulin-beta NRIF3 EMP3 APP DOCK1 Fas LOXL2 Osteoprotegerin MAPT Apolipoprotein E GADD45A PKC,zeta Sphingosine kinase 2 Prostaglandin E R-3 Cell death TRAIL NF-kB GG2-1 Figure 2 A) B) *
Figure 3 OPG Progression of chronic kidney disease Parenchymal renal cellapoptosis TRAIL Inflammation Hyperglycemia