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Preparation for a paper. All notes should be complete such that we can go back and reference them for future writing We want to tell a story so including motivation and plans is important (take the time it will be worth it)
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Preparation for a paper • All notes should be complete such that we can go back and reference them for future writing • We want to tell a story so including motivation and plans is important (take the time it will be worth it) • Preparation of figures and presentation slides along the way will help develop our story and solidify our plans • Limited time necessitate preparing our presentation and any writing we want to do along the way
Image preparation • Existing rasterized images (gels, photos etc.) should be modified (ethically) in photoshop • New drawings or modification of graphs and tables should be done in illustrator • Save in native adobe format as well as other formats for web or integration into writings • Save graphs as .ps or .pdf from graphing program to import into illustrator
More imaging • We will try to keep a standard format for figures as we go so that they can be put together easily • Keep font sizes, line weights, color matching the same throughout image as well as across images • When making images for presentations save as .png with transparency (also save as .ai or .psd)
Labeling and reaction mixing • Label each sample carefully and non-ambiguously before adding reagents • Make a list of reagents for each reaction before starting • check off each reagent as you add them to the reactions • Double check everything is there before incubating or running reaction
Pipetting • Make sure you have the right pipette for the volume needed • When taking fluid depress button of pipette only to the first level of resistance • Look at tube and tip when taking up reagent • When ejecting liquid depress button of pipette all the way down remove from sample and release