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Spot tests to

Spot tests to. spot the cheats. By: INSERT NAME. What is a spot test?. A spot test is … a simple chemical reaction that allows a substance to be identified. Heroin. Cocaine. In drug testing…

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Spot tests to

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  1. Spot tests to spot the cheats By: INSERT NAME

  2. What is a spot test? A spot test is … a simple chemical reaction that allows a substance to be identified. Heroin Cocaine • In drug testing… • a drug or class of drugs gives a characteristic visible change when mixed with another chemical (the reagent) • a sample, such as urine, is tested with the reagent • the presence of the drug (or class of drug) is evidenced by the characteristic visible change; this is a positivetest • if the characteristic change is not seen, it is a negativetest

  3. Now it’s your turn … You have been given three different urine samples from three different athletes: a cyclist (A), an archer (B) and a tennis player (C) A B C • You have two different reagents (labelled Y and Z) • Reagent Y gives a characteristicredcolour if a steroid drug is present • Reagent Z gives a characteristicbluecolour if a beta-blocker is present • Use the reagents to identify which athletes have taken a banned substance

  4. What you have to do Work in pairs. One of you should use the top half of the well plate. The other should repeat the procedure in the bottom half. Place the culture plate on a white piece of paper Mark the columns and rows, as in the diagram Place a drop of urine sample A into the top two wells in column A Place a drop of urine sample B into the top two wells in column B Place a drop of urine sample C into the top two wells in column C Add drops of reagent Y to row Y, and drops of reagent Z to row Z Do your results agree with one another? Which two athletes have taken a banned substance? Which class of banned substance did each athlete take?

  5. Spot tests: the pros They … • are quick and easy to use • are cheap (no complex, expensive equipment needed) • allow selection of samples for further analysis • can be performed outside the lab The colour identifies the class of drug. The intensity of the colour is an approximate measure of how much of the drug is present.

  6. Spot tests: the cons They … • usually only indicate the classof substance (identification of the actual substance requires a different test) • don’t tell you how much of the banned substance is present • don’t give a permanentrecord of the test result Modern instruments can identify the actual drug present, an accurate measure of how much of it is present, and they provide a permanent record.

  7. GlaxoSmithKline and London 2012 By: INSERT NAME

  8. The Olympic Games 26 sports the most recognised brand in the world 10 500 athletes across 205 countries 70 000 volunteers eight million tickets the world’s biggest audience - four billion

  9. The Paralympic Games the second biggest event in the world - audience of 1.8 billion 4200 athletes across 162 countries two million tickets 471 medal events 20 sports

  10. Congrats Team GB! Olympics Paralympics

  11. GSK and London 2012 GlaxoSmithKline Brought Science to the Games … GSK formed a ground-breaking partnership with King’s College London to help make London 2012 the cleanest Games possible … the anti-doping laboratory operated all day, every day 4910 samples were tested for the Olympic Games – 3870 urine and 1040 blood more than 1200 tests were carried out for the Paralympic Games … that’s more tests than any other Games

  12. GSK and London 2012 Official Laboratory Services Provider “ Making London 2012 the fairest Games possible will be crucial in making the Games a success. As a company with science at its heart, GlaxoSmithKline is perfectly placed to provide a world class laboratory, in partnership with King’s College, with the capability to carry out more doping tests than ever before. Science plays an integral role in sport and I hope the London 2012 Games inspires more young people to take up roles in sport science related careers. Lord Seb Coe, Chair of the London Organising Committee of the Olympic Games and Paralympic Games ”

  13. GSK and London 2012 Check out this YouTube channel for videos … GSK Vision London 2012: http://www.youtube.com/playlist?list=PLA2D719CFD3F27508 Visit the Scientists in Sport website for more info … Scientists in Sport: http://www.scientistsinsport.com

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