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My Experiment C o l o r C h r o m a t o g r a p h y

My Experiment C o l o r C h r o m a t o g r a p h y. Presented By Kelsey Leigh Campbell Date: 3 October 2012. What is Chromatography?. Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components.

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My Experiment C o l o r C h r o m a t o g r a p h y

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  1. My ExperimentColorChromatography Presented By Kelsey Leigh Campbell Date: 3 October 2012

  2. What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components.

  3. Uses for Chromatography • Analyze • Identify • Purify • Quantify Chromatography is used by scientists to

  4. Uses for Chromatography • Pharmaceutical Company • Hospital • Law Enforcement • Environmental Agency • Manufacturing Plant Real-life examples of uses for chromatography

  5. Chromatography Defined Detailed Definition: Chromatography is a laboratory technique that separates components within a mixture by using the differential affinities of the components for a mobile medium and for a stationary absorbing medium through which they pass

  6. Chromatography Illustration

  7. Types of Chromatography • Liquid Chromatography • Gas Chromatography • Paper Chromatography • Thin-Layer Chromatography

  8. Principles of Paper Chromatography • Capillary Action – the movement of liquid within the spaces of a porous material due to the forces of adhesion, cohesion, and surface tension. • Solubility – the degree to which a material (solute) dissolves into a solvent.

  9. Overview of the Experiment Purpose To introduce students to the principles and terminology of chromatography and demonstrate separation of the dyes in Sharpies with paper chromatography Time Required Prep Time: 20 minutes Experiment: 45 minutes

  10. Materials List • 6 strips of paper or filter • 6 beakers or jars • All strips must be exactly the same size (e.g., 3 cm × 9 cm) • Different color Sharpies • Volumetric measuring devices • Water • Isopropanol • Pencil • Ruler • Tape

  11. Preparation • Prepare 15 ml of the following isopropanol solutions in appropriately labeled beakers: • 0%, 20%, 50%, 70%, 100% • Cut 5 strips of filter paper • Draw a line 1 cm above the bottom edge of the strip with the pencil • Label each strip with its corresponding solution (color and concentration) • Place a spot from each marker (or other dye sample) on your starting line

  12. Developing the Chromatograms • Place the strips in the beakers • Make sure the solution does not come above your start line • Keep the beakers covered • Let strips develop until the ascending solution front is about 2 cm from the top of the strip • Remove the strips and let them dry

  13. Observing the Chromatograms • Observe how some of the dyes are made up of more than one color • Observe how spots of the same color separated in low concentrations of alcohol compared to higher concentrations • Observe when spots of different colors first started separating in the different concentrations

  14. 0 % 20 % 50 % 70 % 100 %

  15. Black and Blue Separations • Black Dye • Dyes Separated: black and purple • Not soluble in low concentrations of isopropanol • Partially soluble in concentrations >20% • Blue Dye • Dye Separated: blue • Not very soluble in low concentrations • Completely soluble in high concentrations

  16. Green and Red Separations • Green Dye • Dyes Separated: Blue and Yellow • Blue: Soluble in concentrations > 20% • Yellow: Soluble in concentrations > 0% • Red Dye • Dyes Separated: Red and Yellow • Yellow: Soluble in low concentrations and less soluble in high concentrations • Red: Slightly soluble in low concentrations and more soluble in concentrations > 20%

  17. Alternative Experiments • Test Different Samples • Markers, pens, highlighters • Food pigments (Skittles or M&M’s) • Test Different Solvents • Other alcohols: methanol, ethanol, propanol, butanol • Test Different Papers • Coffee Filters • Paper towels • Typing Paper

  18. Questions?!

  19. References • The idea for this experiment came from http://chemistry.about.com/od/chemistryexperiments/ht/candychroma.htm • Scientific Uses for Chromatography http://www.wjec.co.uk/uploads/publications/5403.pdf • Everyday Uses for Chromatography http://www.chacha.com/question/what-are-two-practical-uses-of-chromatography-in-everyday-life http://www.blurtit.com/q6751913.html

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