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Development of a Device for Neurochemical Sample Collection from Freely Moving Monkeys

Development of a Device for Neurochemical Sample Collection from Freely Moving Monkeys. Kara Barnhart Angwei Law Paul Schildgen Grant Smith. Team Members :. Client:. Dr. Ei Terasawa, Dept. of Pediatrics. Advisor:. Professor William Murphy, Dept. of BME. Outline. Client Research

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Development of a Device for Neurochemical Sample Collection from Freely Moving Monkeys

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  1. Development of a Device for Neurochemical Sample Collection from Freely Moving Monkeys Kara Barnhart Angwei Law Paul Schildgen Grant Smith Team Members: Client: Dr. Ei Terasawa, Dept. of Pediatrics Advisor: Professor William Murphy, Dept. of BME

  2. Outline • Client Research • Experimental Setup • Free Monkeys from Chairing • Design Requirements • Solution Ideas • Construction & Testing • Ethical Considerations

  3. Background-Research • Control of growth and development • Brain’s role in triggering puberty • Measures neuropeptides in hypothalamus for neuroendocrine studies • Rhesus monkeys as model system for humans Associated Press. “Scientists Map Genome of Rhesus Monkey” 13 Apr 2007. 4 Mar 2008. <www.foxnews.com/story/0,2933,265907,00.html>.

  4. Background-Experimental Setup • Metal ring implanted into skull • Microdrive unit attached • Monkey transferred to chair • Microdialysis probe inserted • Tubing attached to fraction collector and pump • Monkey confined to chair for 12 hours Forst SI, et al., Microdialysis methods for in vivo neropeptide measurement in the Stalk-median eminence in the Rhesus monkey, J Neurosci Methods (2007), doi: 10.1016/j.jneumeth.2007.09.001 SAEN. The Mary T. and Frank L. Hoffman Family Foundation. 11 Feb 2008. 4 Mar 2008. <www.all-creatures.org/saen/res-fr.html>.

  5. Problem Statement • Construct protective device for microdialysis apparatus • Used during cranial experiments on monkey • Free the monkey from the constraints of chairing • Provide a more comfortable and realistic environment for the monkey • Monkey attached to tether in cage instead of chair

  6. PDS • Client: Design a device to protect the microdrive unit to free monkey from chairing • Safe for monkey • Weight: Under 0.5 kg • Sterile • Safe from monkey • Force: Withstand 100 N • Stable • Detachable

  7. Design Alternative-Jacket • Monkey wears jacket • Metal rod attached • Metal ring around head • Straps from metal ring around chin and neck • PVC pipe covering device

  8. Design Alternative-Helmet • Plastic helmet with straps • Hole at center of helmet • PVC pipe attached to helmet

  9. Design Alternative-Cushion Cylinder • 3 piece aluminum casing • Soft molded polymer core • Straps around chin and neck attached to casing • Secured with an Allen wrench

  10. Design Matrix

  11. Future Work • Figure out exact dimensions • Order materials and parts • Construct prototype • Test device

  12. Ethical Considerations • Research abides by strict federal regulations • Monkeys under constant supervision and care • Monkeys are not harmed or ill-treated during the experiment

  13. Credits • Ei Terasawa • Kim Keen • Professor Murphy • Primate Center Staff

  14. Questions?

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