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European project « 5th PCRDT »

This European project, part of the 5th PCRDT, focuses on the genetic characterization of plantlets through somatic embryogenesis. Objectives include somatic embryogenesis and genetic evaluation of plantlets from embryos. Milestones achieved involve production and germination of embryos, plantlet provision, and more. The research activities include flow cytometry, biochemistry, molecular markers, and analyses. Deliverables include providing the embryogenesis procedure and upcoming Paper DP 5 on garlic plant regeneration. This project involves a scientific and administrative team led by L. Fereol, R. Hugon, and S. Causse.

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European project « 5th PCRDT »

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  1. European project « 5th PCRDT » GARLIC AND HEALTH First annual report Partner P8 CIRAD-FLHOR

  2. General presentation

  3. Scientific and administrative team • L. FEREOL scientist • R. HUGON administrative officer • S. CAUSSE technical assistant

  4. Objectives • Somatic embryogenesis • Genetic characterisation of plantlets from somatic embryos

  5. Research activities

  6. Work plan • Establishment of somatic embryogenesis procedure for three varieties • Rouge Reunion • Messidrome • Morasol • Production and provision to P9 of somatic embryos obtained via callus and culture suspension of these varieties

  7. Work plan (continue) • Genetic evaluation of plants from somatic embryo by : • flow cytometry (by P8) • biochemistry (by P5) • molecular markers (by P1) • morphological and physiological analyses (by P8, P9)

  8. Milestones of the first year done • Production of calli with embryogenic tissue • (cv. Messidrome and Morasol) • Production and germination of embryos from these calli • (cv. Messidrome and Morasol) done

  9. Milestones of the first year(continue) • Provision to P9 of plantlets from somatic embryos via callus • 200 plants from somatic embryos via callus, • cv. Rouge Reunion • cv.Messidrome and Morasol, • Initiation of culture suspension and production of embryos • cv. Rouge Reunion • cv.Messidrome and Morasol, done Partially done done done

  10. Milestones of the first year (continue) • Provision to P9 of plantlets from somatic embryos via culture suspension • 200 plants from somatic embryos via culture suspension, • cv. Rouge Reunion • cv.Messidrome and Morasol, done Partially done

  11. What has been done • CV. ROUGE DE LA REUNION • Production of embryos from embryogenic calli on semi solid medium and from cell suspension • Germination of these embyos • in vitro development of one part of these embryos • Provision to P9 of the other part to continue in vitro development • Provision to P9 of embryo plantlets for acclimatation and field evaluation

  12. What has been done • MESSIDROME and MORADO DE CUENCA (MORASOL) • Culture initiation on march 2000 for embryogenic callus induction • Adaptation of the technique of embryogeneic callus induction to these cv. • Histological studies of the calli • Obtention of embryogenic calli • Maintenance and multiplication of the material

  13. What has been done • Supprimez les exemples d'icônes de documentet remplacez-les par les icônes des documents actifs en procédant comme suit: • Dans le menu Insertion, cliquez sur Objet... • Cliquez sur “Objet existant” • Recherchez le nom du fichier dans la zone “Fichier” • Assurez-vous que la case à cocher “Afficher sous forme d'icône” est activée • Cliquez sur OK • Sélectionnez une icône • Dans le menu Diaporama, cliquez sur “Paramètres des actions” • Cliquez sur “Action OLE” et sélectionnez “Modifier” • Cliquez sur OK • MESSIDROME and MORADO DE CUENCA (MORASOL) (continue) • Subculturing embrylogenic tissue on embryo producing medium • Studies are carry on for adaptation of the produre to establlish cell suspension from embryogenic calli

  14. Deliverables • Embryogenesis procedure provided to G&H partners • Paper DP 5: Regeneration and acclimatisation of garlic plants issued from embryogenic callus (P8, P9, in progress) In progress, submitted july 2001

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