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Contribution of the University of Freiburg

The Ca rbon Si nk Strength of Beech in a Changing Environment: Experimental R isk Assessment of Mitigation by Chronic Oz one Impact (CASIROZ). Contribution of the University of Freiburg.

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Contribution of the University of Freiburg

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  1. The Carbon Sink Strength of Beech in a Changing Environment: Experimental Risk Assessment of Mitigation by Chronic Ozone Impact (CASIROZ) Contribution of the University of Freiburg Prof. Dr. H. Rennenberg Dr. K. Haberer, M. Alexou (participant 4, WP1) Albert-Ludwigs-Universität Freiburg i.Br.Institut für Forstbotanik und BaumphysiologieGeorges-Köhler-Allee D-79085 Freiburg In association:PD Dr. C. Langebartels (subcontractor 2, WP2)Institute for Biochemical Plant PathologyGSF-Forschungszentrum für Umwelt und GesundheitD-85764 Oberschleißheim

  2. GENERAL PUBLIC ENVIRONMENTAL POLICIES DECISION MAKING WP 2 3 CR Sub- contractor1 1 CO 2 AC WP 1 6 CR 4 CR 8CR 7CR 5CR Sub-contractor 2 Project structure

  3. Transpiration, stomatal conductance, photosynthesis Relative activity changes between carbon fixing enzymes Control of carbon fixation at the gene level Stem production, xylem mass flow through the trunk C and N allocation in phloem and xylem (cycling of carbohydrates, N-Status) Fine-root functionality (H2O acquisitation) Identification of different types of ectomycorrhiza Fumigation experiments in cuvettes Exposure of young beech plants in the canopy Production of defense metabolites in leaves: Antioxidants and redox status Injury amplification reactions Regulation of defense reactions at the gene level Genetic regulation of Rubisco, PEPC Production of defense metabolites in roots Examination of chromosomes Phytohormonal relationships in leaves, roots, phloem, xylem Extent of mycorrhization Examination of 3-year-old beech plants Workpackage description WP 1Ecophysiology and modeling WP 2Biochemistry and molecular biology MODELING

  4. Aims of the project –part of CR 4/sub-c 2- Effects of chronic O3-exposure on Activity of carbon-fixing enzymes Level of sugars Antioxidative system Phytohormons Injury amplification reactions Cycling of carbohydrates Fine-root functionality N-Status

  5. Hypotheses • Risk of O3 injury to processes of primary metabolism under light limitation is as high as under sunlight. • The symptom development in beech leaves as a consequence of O3 exposure are determined by the antioxidant status and damage amplifying reactions. • Effects of chronic O3 exposure on processes of primary metabolism are different in young and adult trees and cuvette fumigation experiments. • Chronic O3 impact constrains the carbon sink strength.

  6. Role of participant 4 and sub-contractor 2

  7. Methods • Examination of carbon-fixing enzymes 12C/13C and 16O/18O IR-MS ( water-use-efficiency) (Isotope Ratio Mass Spectrometry) • Ascorbate High Pressure Liquid Chromatography (HPLC) • Glutathione High Pressure Liquid Chromatography (HPLC) • Sugar concentration High Pressure Liquid Chromatography (HPLC) • N-status/translocation xylem-phloem TSSN-analysis14N/15N (IR-MS) • Fine root functionality (H2O-acquisitation) 16O/18O (IR-MS)

  8. Time table march 2003 march 2004 march 2005 Dataset adult and young trees (1st growing season) Dataset adult and young trees (2nd growing season) Methodological preparations Field assessment:Sampling, analysing leaves, xylem, phloem, stem wood, soil Data integration

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