Pyrabactin Mimicking Plant Hormone Interactions in Membrane Signaling Pathways

Supplementary Figure 1: b PYL5 Marker PYL4 PYL6 PYR1 PYL1 PYL2 PYL3 a None PYL2 PYL3 PYL4 PYL5 PYL6 PYR1 PYL1 c apo-PYL2 ABA-PYL2 apo-PYL1 ABA-PYL2 -HAB1 None PYL2 PYL3 PYL4 PYL5 PYL6 PYR1 PYL1 d HAB1 Mg2+ PYL2 pre- post- gelfiltration column None PYL2 PYL3 PYL4 PYL5 PYL6 PYR1 PYL1

Supplementary Figure 2: N N a C C gate gate latch latch b C N N C

Supplementary Figure 3: a K64 K64 N173 N173 E98 E98 E147 E147 b c latch + gate ABA-PYL2 apo-PYL2 ABA * ABA PYL2 G246D Mg2+ Mg2+ HAB1/PP2C HAB1/PP2C

Supplementary Figure 4: a 680 nm 520-620 nm Singlet Oxygen b Streptavidin -Coated Donor Bead Ni-coated Acceptor Bead PYL2-H6 b-HAB1 IC50 = 250nM + untagged PYL2 competitor PYL2 680 nm Singlet Oxygen X no signal Streptavidin Ni-coated Acceptor Bead b-HAB1 PYL2 PYL2-H6

Supplementary Figure 5: ligand binding pocket gate relative luciferase activity latch

Supplementary Figure 6: a PYR1 residue number gate latch c b d

Pyrabactin Mimicking Plant Hormone Interactions in Membrane Signaling Pathways

Pyrabactin Mimicking Plant Hormone Interactions in Membrane Signaling Pathways

Presentation Transcript

Supplementary Figure S1

supplementary Figure 1 .

Supplementary Figure 1

Supplementary Figure S7

Supplementary Figure 2.

Supplementary figure 4

Supplementary figure 1

a) S1-05

Supplementary figure S1

Supplementary Figure S1

Supplementary figure 2

Supplementary Figure 1. Suzuki et al.

Supplementary Figure 1

Supplementary Figure 1

Retention time (min)

Supplementary Figures

YPD

Supplementary Figure 2. Monocytes purity and expression of TLRs

Supplementary Figure 1

s12968-014-0064-2-s2

Voso et al, Supplementary Figure 1