1 / 2

Student work stations for pGLO lab

Student work stations for pGLO lab. CaCl. Each student group will need four tubes. Label all tubes on the lid/top Label Tube 1 “LB” (for Luria- Bertania Broth) fill with 1mL of LB Label Tube 2 “ CaCl ” (for Calcium Chloride Sol’n ) Fill with 1mL of CaCl Label Tube 3 “+ DNA”

ekram
Download Presentation

Student work stations for pGLO lab

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Student work stations for pGLO lab CaCl • Each student group will need four tubes. Label all tubes on the lid/top • Label Tube 1 “LB” (for Luria-BertaniaBroth) • fill with 1mL of LB • Label Tube 2 “CaCl” (for Calcium Chloride Sol’n) • Fill with 1mL of CaCl • Label Tube 3 “+ DNA” • this tube gets the plasmid and is the “experimental chamber” • Label Tube 4 “- DNA” • This tube doesn’t receive plasmid and is the “control chamber” • Place all four tubes in that “floatie” • but if you have microfuge tube racks, use them. • You can choose your own “color coding” system • View from above: LB +DNA -DNA

  2. Schematic Diagram of control (-DNA)and experimental (+DNA) tubes 3) 250 ul LB 3) 250 ul LB 2a) one Colony of E. coli 2) one Colony of E. coli 2b) 8-10 ul pGLO plasmid 1) 250ul CaCL 1) 250ul CaCL -DNA (total volume = 500 ul*) +DNA (total volume 510 ul*) *aliquot 100 ul of tube contents onto the appropriate plate and spread. Follow protocol.

More Related