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pGLO

pGLO. Bacterial Genetics. E. Coli structure. E. Coli structure. E. Coli structure. E. Coli structure. E. Coli structure. E. Coli structure. E. Coli structure. E. Coli structure. Plasmid. Additional DNA found in some bacteria Small loop Carries one or more genes

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pGLO

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  1. pGLO Bacterial Genetics

  2. E. Coli structure

  3. E. Coli structure

  4. E. Coli structure

  5. E. Coli structure

  6. E. Coli structure

  7. E. Coli structure

  8. E. Coli structure

  9. E. Coli structure

  10. Plasmid • Additional DNA found in some bacteria • Small loop • Carries one or more genes • Can be transferred from one bacteria to another.

  11. pGLO plasmid (original form) • ori- point of origin for DNA replication • araC- gene for repressor protein in arabinose operon • araB, A and D- gene for arabinose metabolism ara B, ara A & ara D

  12. pGLO plasmid (engineered form) • Gene for arabinose metabolism (ara BAD) has been replaced with GFP. • Amipicillin resistance has been added (bla)

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