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Developing a database of identified neurons for the mollusc, Tritonia

Developing a database of identified neurons for the mollusc, Tritonia. C2. DSI. 50mV. VSI. 10s. Photo by Bill Frost. Characteristics for Uniquely Identifying Neurons. Neuroanatomy Soma location and characteristics Neurochemical identity (immunohistochemistry)

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Developing a database of identified neurons for the mollusc, Tritonia

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  1. Developing a database of identified neurons for the mollusc, Tritonia

  2. C2 DSI 50mV VSI 10s Photo by Bill Frost

  3. Characteristics for Uniquely Identifying Neurons • Neuroanatomy • Soma location and characteristics • Neurochemical identity (immunohistochemistry) • Axon and dendrite branching pattern • Electrophysiology • Membrane & firing properties • Synaptic connectivity • Pharmacology

  4. Relational Diagram

  5. (1,1) (0,N) Update Log (1,1) (1,1) XMLStore XMLfile (1,1) (1,1) TextStore TextFile (1,1) (1,1) (0,1) (0,1) Has Soma Belong Soma Cluster (0,N) (0,N) GLocate Primary (0,N) (1,N) (1,N) (1,1) IsAt Pass Section Ganglion (1,1) (0,1) (0,N) (0,N) (0,N) GLocate Has Axons Project Axons Nerve (1,2) Neuron (0,N) (0,N) hasMolecule Molecule (0,1) (0,N) (0,N) (1,1) Conduct HasEP (2,2) Conductance Electrophysiology (0,N) (0,N) Connect FP Firing Pattern ElectricalSynapse (0,1) Neuromodulation (1,N) (0,N) Connections U HasComponent Component ChemicalSynapse NegativeConnection “Simplified” Entity-Relationship Diagram

  6. Update Log XMLStore XMLfile TextStore TextFile Has Soma Belong Soma Cluster GLocate Primary IsAt Pass Section Ganglion GLocate Has Axons Project Axons Nerve Neuron hasMolecule Molecule Conduct HasEP Conductance Electrophysiology Connect FP Firing Pattern ElectricalSynapse Neuromodulation Connections U HasComponent Component ChemicalSynapse NegativeConnection “Simplified” Entity-Relationship Diagram

  7. C2 Update Log XMLStore XMLfile TextStore TextFile Has Soma Belong Soma Cluster GLocate Primary IsAt Pass Section Ganglion GLocate Has Axons Project Axons Nerve Neuron hasMolecule Molecule Conduct HasEP Conductance Electrophysiology Connect FP Firing Pattern ElectricalSynapse Neuromodulation Connections U HasComponent Component ChemicalSynapse NegativeConnection Neurons can be identified by soma location and characteristics DSIs

  8. Update Log XMLStore XMLfile TextStore TextFile Has Soma Belong Soma Cluster GLocate Primary IsAt Pass Section Ganglion GLocate Has Axons Project Axons Nerve Neuron hasMolecule Molecule Conduct HasEP Conductance Electrophysiology Connect FP Firing Pattern ElectricalSynapse Neuromodulation Connections U HasComponent Component ChemicalSynapse NegativeConnection The DSIs can be identified by serotonin immunoreactivity Soma position can be variable 200 m

  9. Update Log XMLStore XMLfile TextStore TextFile Has Soma Belong Soma Cluster GLocate Primary IsAt Pass Section Ganglion GLocate Has Axons Project Axons Nerve Neuron hasMolecule Molecule Conduct HasEP Conductance Electrophysiology Connect FP Firing Pattern ElectricalSynapse Neuromodulation Connections U HasComponent Component ChemicalSynapse NegativeConnection VSI-B C2 DSI Branching pattern does not provide much information. We need to store a rough estimate of the projection pattern

  10. Update Log XMLStore XMLfile TextStore TextFile Has Soma Belong Soma Cluster GLocate Primary IsAt Pass Section Ganglion GLocate Has Axons Project Axons Nerve Neuron hasMolecule Molecule Conduct HasEP Conductance Electrophysiology Connect FP Firing Pattern ElectricalSynapse Neuromodulation Connections U HasComponent Component ChemicalSynapse NegativeConnection Neurons can be distinguished by which nerve their axons project through

  11. Update Log XMLStore XMLfile TextStore TextFile Has Soma Belong Soma Cluster GLocate Primary IsAt Pass Section Ganglion GLocate Has Axons Project Axons Nerve Neuron hasMolecule Molecule Conduct HasEP Conductance Electrophysiology Connect FP Firing Pattern ElectricalSynapse Neuromodulation Connections U HasComponent Component ChemicalSynapse NegativeConnection Neurons can be identified by spike shape and spontaneous activity DSI-1 50mV DSI-2 Neuron 3 Neuron 4 1s

  12. Update Log XMLStore XMLfile TextStore TextFile Has Soma Belong Soma Cluster GLocate Primary IsAt Pass Section Ganglion GLocate Has Axons Project Axons Nerve Neuron hasMolecule Molecule Conduct HasEP Conductance Electrophysiology Connect FP Firing Pattern ElectricalSynapse Neuromodulation Connections U HasComponent Component ChemicalSynapse NegativeConnection Neurons can be identified by synaptic inputs and outputs

  13. Update Log XMLStore XMLfile TextStore TextFile Has Soma Belong Soma Cluster GLocate Primary IsAt Pass Section Ganglion GLocate Has Axons Project Axons Nerve Neuron hasMolecule Molecule Conduct HasEP Conductance Electrophysiology Connect FP Firing Pattern ElectricalSynapse Neuromodulation Connections U HasComponent Component ChemicalSynapse NegativeConnection Synaptic interactions can be complex

  14. Characteristics for Uniquely Identifying Neurons • Neuroanatomy • Soma location and characteristics • Neurochemical identity (immunohistochemistry) • Axon and dendrite branching pattern • Electrophysiology • Membrane & firing properties • Synaptic connectivity • Pharmacology

  15. Homologous neurons can be recognized in closely related species Tritonia diomedea Melibe leonina DSIs CSPs

  16. Features that we want in our database • Simple design • Web based • Easy to enter data • Easy to keep up-to-date • Exemplars of data rather than all raw data • High quality of data • Refereed input • Completely annotated with sources • Citable

  17. A tool for identifying neurons • Use a separate database to gather individual observations • Private, within lab use only • Flag entries that resemble each other • Coalesce to form Canonical description

  18. www.biology.gsu.edu/neurosci • Identified Neuron Database • Katz Lab members • James Newcomb • Robert Calin-Jageman, Ph.D. • GSU Computer Science • Raj Sunderraman, Ph.D. • Hao Tian • Christopher Gardner • Ying Zhu, Ph.D. • Jason Pamplin • Supported by • NIH / NINDS • Brains & Behavior Program • Center for Behavioral Neuroscience • GSU RPE award

  19. Simplified Entity-Relationship Diagram

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