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Microscopy

Microscopy. Introduction of Microscopes. Microscopic – invisible to naked eye 1595 Magnified images approximately 3x – 10x. Compound Microscopes. Uses natural or artificial light to see objects. Parts of a Compound Microscope. Ocular lens Objective lens Low, Medium, and High power Body

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Microscopy

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  1. Microscopy

  2. Introduction of Microscopes • Microscopic – invisible to naked eye • 1595 • Magnified images approximately 3x – 10x

  3. Compound Microscopes • Uses natural or artificial light to see objects

  4. Parts of a Compound Microscope • Ocular lens • Objective lens • Low, Medium, and High power • Body • Arm • Base • Focus • Coarse adjustment • Fine adjustment • Stage • Stage clip • Stage controls • Light source • Diaphragm

  5. Handling Microscope • Carry the microscope with BOTH hands – one underneath the Base and one on the Arm. • Always store it with the Low Power Objective Lens above the Diaphragm.

  6. Total Magnification • Ocular power x objective power = Total Magnification

  7. Field of View • Measured in µm • Can be measured using ruler or graph paper

  8. Preparing Wet Mounts • Normally use water • Starting at an angle, place coverslip gently on the specimen. • NO AIR BUBBLES

  9. Electron Microscopes • Uses electrons instead of light • Transmission Electron Microscope • Allows user to see through object • Scanning Electron Microscope • Allows user to see the surface of object

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